Evidence map›Paper›PMID 34360885›Full record

ArticleInternational journal of molecular sciences2021

Core Histones Are Constituents of the Perinuclear Theca of Murid Spermatozoa: An Assessment of Their Synthesis and Assembly during Spermiogenesis and Function after Gametic Fusion.

Lauren E Hamilton, Morgan Lion, Luis Aguila, João Suzuki, Genevieve Acteau, Nicole Protopapas, Wei Xu, Peter Sutovsky, Mark Baker, Richard Oko

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Lauren E HamiltonDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Morgan LionDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Luis AguilaCenter for Research in Reproduction and Fertility, Department of Veterinary Sciences, Université de Montreal, St. Hyacinthe, QC J2S 2M2, Canada.ORCID 0000-0001-9713-4248
João SuzukiCenter for Research in Reproduction and Fertility, Department of Veterinary Sciences, Université de Montreal, St. Hyacinthe, QC J2S 2M2, Canada.
Genevieve ActeauDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Nicole ProtopapasDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Wei XuDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Peter SutovskyDivision of Animal Science, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO 65211, USA.ORCID 0000-0002-9231-2823
Mark BakerSchool of Environmental and Life Science, University of Newcastle, Callaghan, NSW 2308, Australia.
Richard OkoDepartment of Biomedical and Molecular Sciences, Queen's University, Botterell Hall, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.
Queen's University · CAUniversité de Montréal · CAUniversity of Missouri Health System · USUniversity of Newcastle Australia · AU

Funding

CIHR 84440Natural Sciences and Engineering Research Council of Canada RGPIN/05305USDA National Institute of Food and Agriculture 2015-67015-23231
6 · The paper itself

Abstract

The perinuclear theca (PT) of the eutherian sperm head is a cytoskeletal-like structure that houses proteins involved in important cellular processes during spermiogenesis and fertilization. Building upon our novel discovery of non-nuclear histones in the bovine PT, we sought to investigate whether this PT localization was a conserved feature of eutherian sperm. Employing cell fractionation, immunodetection, mass spectrometry, qPCR, and intracytoplasmic sperm injections (ICSI), we examined the localization, developmental origin, and functional potential of histones from the murid PT. Immunodetection localized histones to the post-acrosomal sheath (PAS) and the perforatorium (PERF) of the PT but showed an absence in the sperm nucleus. MS/MS analysis of selectively extracted PT histones indicated that predominately core histones (i.e., H3, H3.3, H2B, H2A, H2AX, and H4) populate the murid PT. These core histones appear to be

Indexed as

AnimalsCell NucleusChromatography, LiquidFemaleFertilizationHistonesMaleMiceMice, Inbred C57BLRatsRats, Sprague-DawleySpermatidsSpermatogenesisSperm HeadSperm Injections, IntracytoplasmicTandem Mass SpectrometryHistonesfertilizationgamete biologyhistonesICSImass spectrometrymicrotubular manchetteperforatoriumperinuclear thecapostacrosomal sheathspermatozoaspermiogenesis

Identifiers

PMID34360885
PMCPMC8347300
OpenAlexW3183983711

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.