ArticleInternational journal of molecular sciences2021
Interleukin-4 Promotes Tuft Cell Differentiation and Acetylcholine Production in Intestinal Organoids of Non-Human Primate.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Stem cell-based tactics in the remodeling and treatment of intestinal diseases.Stem cell research & therapy · 2026Review
- Rank-dependent control of tuft and BEST4 cell development in the intestine.Nature communications · 2026Article
- Generation and functional characterization of tuft cells in non-human primate pancreatic ducts through organoid culture systems.Frontiers in cell and developmental biology · 2025Article
- Acetylcholine from tuft cells promotes M2 macrophages polarization in Hirschsprung-associated enterocolitis.Frontiers in immunology · 2025Article
- Paraffin Embedding and Histological Staining of Intestinal Organoids.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Intestinal Tuft Cells Are Enriched With Protocadherins.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Azoxymethane-induced carcinogenesis-like model of mouse intestine and mouse embryonic stem cell-derived intestinal organoids.Molecular biology reports · 2024Article
- Intestinal Tuft Cells: Morphology, Function, and Implications for Human Health.Annual review of physiology · 2024Review
- Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm.International journal of molecular sciences · 2024Review
- Diet-mediated constitutive induction of novel IL-4+ ILC2 cells maintains intestinal homeostasis in mice.The Journal of experimental medicine · 2023Article
- Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lungFrontiers in toxicology · 2023Review
- Possible connection between intestinal tuft cells, ILC2s and obesity.Frontiers in immunology · 2023Review
- "Every cell is an immune cell; contributions of non-hematopoietic cells to anti-helminth immunity".Mucosal immunology · 2022Review
- Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces.PLoS pathogens · 2022Review
- Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
In the intestine, the innate immune system excludes harmful substances and invading microorganisms. Tuft cells are taste-like chemosensory cells found in the intestinal epithelium involved in the activation of group 2 innate lymphoid cells (ILC2). Although tuft cells in other tissues secrete the neurotransmitter acetylcholine (ACh), their function in the gut remains poorly understood. In this study, we investigated changes in the expression of genes and cell differentiation of the intestinal epithelium by stimulation with interleukin-4 (IL-4) or IL-13 in macaque intestinal organoids. Transcriptome analysis showed that tuft cell marker genes were highly expressed in the IL-4- and IL-13-treated groups compared with the control, and the gene expression of choline acetyltransferase (ChAT), a synthesis enzyme of ACh, was upregulated in IL-4- and IL-13-treated groups. ACh accumulation was observed in IL-4-induced organoids using high-performance liquid chromatography-mass spectrometry (HPLC/MS), and ACh strongly released granules from Paneth cells. This study is the first to demonstrate ACh upregulation by IL-4 induction in primates, suggesting that IL-4 plays a role in Paneth cell granule secretion via paracrine stimulation.
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Registered trials
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