ArticleInternational journal of molecular sciences2021
Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Impact of aging on gut-lung-adipose tissue interactions and lipid metabolism during influenza infection in mice.Scientific reports · 2025Article
- Luteolin Induces Nrf2 Activity in C2C12 Cells: Implications for Muscle Health.International journal of molecular sciences · 2025Article
- Article
- Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model.International journal of molecular sciences · 2024Article
- Heterogeneity of immune cells and their communications unveiled by transcriptome profiling in acute inflammatory lung injury.Frontiers in immunology · 2024Article
- Article
- Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury.Methods and protocols · 2023Article
- Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks.Frontiers in veterinary science · 2023Article
- Physiological Mineralization during In Vitro Osteogenesis in a Biomimetic Spheroid Culture Model.Cells · 2022Article
- A juvenile murine model with chronic lung inflammation induced by repeated intratracheal instillation of lipopolysaccharides: a versatile and replicable model.Translational pediatrics · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
qRT-PCR still remains the most widely used method for quantifying gene expression levels, although newer technologies such as next generation sequencing are becoming increasingly popular. A critical, yet often underappreciated, problem when analysing qRT-PCR data is the selection of suitable reference genes. This problem is compounded in situations where up to 25% of all genes may change (e.g., due to leukocyte invasion), as is typically the case in ARDS. Here, we examined 11 widely used reference genes for their suitability in commonly used models of acute lung injury (ALI): ventilator-induced lung injury (VILI), in vivo and ex vivo, lipopolysaccharide plus mechanical ventilation (MV), and hydrochloric acid plus MV. The stability of reference gene expression was determined using the NormFinder, BestKeeper, and geNorm algorithms. We then proceeded with the geNorm results because this is the only algorithm that provides the number of reference genes required to achieve normalisation. We chose interleukin-6 (
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