Evidence map›Paper›PMID 34360609›Full record

ArticleInternational journal of molecular sciences2021

Soluble Expression and Efficient Purification of Recombinant Class I Hydrophobin DewA.

Sang-Oh Ahn, Ho-Dong Lim, Sung-Hwan You, Dae-Eun Cheong, Geun-Joong Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Microorganisms · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Sang-Oh AhnDepartment of Biological Sciences, College of Natural Sciences, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Ho-Dong LimCenter for Industrialization of Agricultural and Livestock Microorganisms, 241 Cheomdangwahak-ro, Jeongeup-si 56212, Jeollabuk-do, Korea.
Sung-Hwan YouBiomedical Research Center, Chonnam National University, Convergence Science Building (M2), Suite 301-1 264, Seoyang-ro, Hwasun-eup, Hwasun-gun 58128, Jeollanam-do, Korea.
Dae-Eun CheongDepartment of Biological Sciences, College of Natural Sciences, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Geun-Joong KimDepartment of Biological Sciences, College of Natural Sciences, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Chonnam National University · KRChonnam National University Hwasun Hospital · KRJeonbuk Agricultural Research & Extension Services · KR

Funding

the Ministry of Oceans and Fisheries, Korea 20170305
6 · The paper itself

Abstract

Hydrophobins are small proteins (<20 kDa) with an amphipathic tertiary structure that are secreted by various filamentous fungi. Their amphipathic properties provide surfactant-like activity, leading to the formation of robust amphipathic layers at hydrophilic-hydrophobic interfaces, which make them useful for a wide variety of industrial fields spanning protein immobilization to surface functionalization. However, the industrial use of recombinant hydrophobins has been hampered due to low yield from inclusion bodies owing to the complicated process, including an auxiliary refolding step. Herein, we report the soluble expression of a recombinant class I hydrophobin DewA originating from

Indexed as

Aspergillus nidulansFungal ProteinsHydrophobic and Hydrophilic InteractionsRecombinant ProteinsSurface PropertiesDEWA protein, Asperigillus nidulansFungal ProteinsRecombinant Proteinsaqueous two-phase separation (ATPS)isopropyl alcohol (IPA)ramp tag (RT)recombinant hydrophobin DewAsoluble expression

Identifiers

PMID34360609
PMCPMC8345945
OpenAlexW3183651720

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.