Evidence map›Paper›PMID 34359838›Full record

ArticleCells2021

Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes.

Jae Kyun Park, Ju Hee Lee, Eun A Park, Hyunjung J Lim, Sang Woo Lyu, Woo Sik Lee, Jayeon Kim, Haengseok Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jae Kyun ParkDepartment of Biomedical Sciences, CHA University, Seongnam 13488, Korea.ORCID 0000-0002-5333-4990
Ju Hee LeeDepartment of Biomedical Sciences, CHA University, Seongnam 13488, Korea.ORCID 0000-0001-8124-0361
Eun A ParkCHA Fertility Center Seoul Station, CHA University, Seoul 04637, Korea.
Hyunjung J LimDepartment of Veterinary Medicine, School of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.ORCID 0000-0003-2191-666X
Sang Woo LyuCHA Fertility Center Gangnam, CHA University, Seoul 06125, Korea.
Woo Sik LeeCHA Fertility Center Gangnam, CHA University, Seoul 06125, Korea.
Jayeon KimCHA Fertility Center Seoul Station, CHA University, Seoul 04637, Korea.
Haengseok SongDepartment of Biomedical Sciences, CHA University, Seongnam 13488, Korea.

Funding

Korea Health Industry Development Institute HI18C1837National Research Foundation of Korea NRF-2019R1A6A1A03032888
6 · The paper itself

Abstract

The open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and developmental competence of vitrified/warmed (VW) oocytes using the CC were significantly lower than with OC. We aimed to improve the efficiency of the CC. Metaphase II oocytes were collected from mice after superovulation and subjected to in vitro fertilization after vitrification/warming. Increasing the cooling/warming rate and exposure time to cryoprotectants as key parameters for the CC effectively improved the survival rate and developmental competence of VW oocytes. When all the conditions that improved the outcomes were applied to the conventional CC, hereafter named the modified vitrification/warming procedure using CC (mVW-CC), the viability and developmental competence of VW oocytes were significantly improved as compared to those of VW oocytes in the CC. Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future.

Indexed as

VitrificationAnimalsBiomarkersBlastocystCadherinsCDX2 Transcription FactorCells, CulturedCell SurvivalCryopreservationCryoprotective AgentsDimethyl SulfoxideEthylene GlycolFemaleFertilization in VitroGene ExpressionMaleBiomarkersCadherinsCdh1 protein, mouseCdx2 protein, mouseCDX2 Transcription FactorCryoprotective AgentsDimethyl SulfoxideEthylene GlycolSucroseclosed carrier systemscooling ratecross-contaminationdevelopmental competenceexposure time to cryoprotectantsoocyte vitrificationviabilitywarming rate

Identifiers

PMID34359838
PMCPMC8304188

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.