Evidence map›Paper›PMID 34359688›Full record

ArticleCancers2021

Electrochemical Detection of Global DNA Methylation Using Biologically Assembled Polymer Beads.

Narshone Soda, Zennia Jean Gonzaga, Amandeep Singh Pannu, Navid Kashaninejad, Richard Kline, Carlos Salomon, Nam-Trung Nguyen, Prashant Sonar, Bernd H A Rehm, Muhammad J A Shiddiky

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Recent Advances in the Electrochemical Biosensing of DNA Methylation.International journal of molecular sciences · 2025
    Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Narshone SodaSchool of Environment and Science (ESC), Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.
Zennia Jean GonzagaCentre for Cell Factories and Biopolymers (CCFB), Griffith Institute for Drug Discovery (GRIDD), Griffith University, Nathan, QLD 4111, Australia.
Amandeep Singh PannuCentre for Material Science, School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4001, Australia.
Navid KashaninejadQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.ORCID 0000-0002-4385-7771
Richard KlineDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.
Carlos SalomonMaternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.ORCID 0000-0003-4474-0046
Nam-Trung NguyenQueensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.ORCID 0000-0003-3626-5361
Prashant SonarCentre for Material Science, School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD 4001, Australia.ORCID 0000-0002-1119-4897
Bernd H A RehmCentre for Cell Factories and Biopolymers (CCFB), Griffith Institute for Drug Discovery (GRIDD), Griffith University, Nathan, QLD 4111, Australia.
Muhammad J A ShiddikySchool of Environment and Science (ESC), Griffith University, Nathan Campus, Nathan, QLD 4111, Australia.
Griffith University · AUQueensland University of Technology · AUOchsner Medical Center · USThe University of Queensland · AU

Funding

Australian Research Council DP180100055Australian Research Council DP190102944Griffith University International Postgraduate Research Scholarship GUIPRS
6 · The paper itself

Abstract

DNA methylation is a cell-type-specific epigenetic marker that is essential for transcriptional regulation, silencing of repetitive DNA and genomic imprinting. It is also responsible for the pathogenesis of many diseases, including cancers. Herein, we present a simple approach for quantifying global DNA methylation in ovarian cancer patient plasma samples based on a new class of biopolymer nanobeads. Our approach utilises the immune capture of target DNA and electrochemical quantification of global DNA methylation level within the targets in a three-step strategy that involves (i) initial preparation of target single-stranded DNA (ss-DNA) from the plasma of the patients' samples, (ii) direct adsorption of polymer nanobeads on the surface of a bare screen-printed gold electrode (SPE-Au) followed by the immobilisation of 5-methylcytosine (5mC)-horseradish peroxidase (HRP) antibody, and (iii) immune capture of target ss-DNA onto the electrode-bound PHB/5mC-HRP antibody conjugates and their subsequent qualification using the hydrogen peroxide/horseradish peroxidase/hydroquinone (H

Indexed as

DNA methylationelectrochemical detectionovarian cancerpolyhydroxybuytrate nanobeads

Identifiers

PMID34359688
PMCPMC8345084
OpenAlexW3184871519

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.