Evidence map›Paper›PMID 34358999›Full record

ArticleBiosensors & bioelectronics2021

Point-of-need detection of pathogen-specific nucleic acid targets using magnetic particle spectroscopy.

Enja Laureen Rösch, Jing Zhong, Aidin Lak, Zhe Liu, Markus Etzkorn, Meinhard Schilling, Frank Ludwig, Thilo Viereck, Birka Lalkens

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Enja Laureen RöschInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Jing ZhongInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Aidin LakInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Zhe LiuInstitute of Applied Physics, TU Braunschweig, Mendelssohnstraße 2, 38106, Braunschweig, Germany.
Markus EtzkornInstitute of Applied Physics, TU Braunschweig, Mendelssohnstraße 2, 38106, Braunschweig, Germany.
Meinhard SchillingInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Frank LudwigInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Thilo ViereckInstitute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
Birka LalkensInstitute of Semiconductor Technology and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Langer Kamp 6a/b, 38106, Braunschweig, Germany. Electronic address: b.lalkens@tu-braunschweig.de.
Technische Universität Braunschweig · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ongoing COVID-19 pandemic stresses the need for widely available diagnostic tests for the presence of SARS-CoV-2 in individuals. Due to the limited availability of vaccines, diagnostic assays which are cheap, easy-to-use at the point-of-need, reliable and fast, are currently the only way to control the pandemic situation. Here we present a diagnostic assay for the detection of pathogen-specific nucleic acids based on changes of the magnetic response of magnetic nanoparticles: The target-mediated hybridization of modified nanoparticles leads to an increase in the hydrodynamic radius. This resulting change in the magnetic behaviour in an ac magnetic field can be measured via magnetic particle spectroscopy (MPS), providing a viable tool for the accurate detection of target nucleic acids. In this work we show that single stranded DNA can be detected in a concentration-dependent manner by these means. In addition to detecting synthetic DNA with an arbitrary sequence in a concentration down to 500 pM, we show that RNA and SARS-CoV-2-specific DNA as well as saliva as a sample medium can be used for an accurate assay. These proof-of-principle experiments show the potential of MPS based assays for the reliable and fast diagnostics of pathogens like SARS-CoV-2 in a point-of-need fashion without the need of complex sample preparation.

Indexed as

Biosensing TechniquesCOVID-19Nucleic AcidsHumansMagnetic PhenomenaPandemicsRNA, ViralSARS-CoV-2Sensitivity and SpecificitySpectrum AnalysisNucleic AcidsRNA, ViralDiagnosticsMagnetic nanoparticlesMagnetic particle spectroscopyPoint-of-needSARS-CoV-2

Identifiers

PMID34358999
PMCPMC8314793
OpenAlexW3185417509

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.