ArticleVeterinary sciences2021
Accessing Dietary Effects on the Rumen Microbiome: Different Sequencing Methods Tell Different Stories.
Article in Veterinary sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Chemical Characterization and Rumen-Modulating Effects ofMolecules (Basel, Switzerland) · 2026Article
- Effect of Camelina sativa seeds on rumen microbiota and fermentation in dairy sheep.Journal of animal science · 2026Article
- Associations between hyperketonemia and the rumen metagenome and metabolome in Holstein cows during the first 2 weeks postpartum.Animal microbiome · 2025Article
- Retrospective analysis of antimicrobial resistance associated with bovine respiratory disease.Applied and environmental microbiology · 2025Article
- Article
- Distinctive roles between rumen epimural and content bacterial communities on beef cattle feed efficiency: A combined analysis.Current research in microbial sciences · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The current study employed both amplicon and shotgun sequencing to examine and compare the rumen microbiome in Angus bulls fed with either a backgrounding diet (BCK) or finishing diet (HG), to assess if both methods produce comparable results. Rumen digesta samples from 16 bulls were subjected for microbial profiling. Distinctive microbial profiles were revealed by the two methods, indicating that choice of sequencing approach may be a critical facet in studies of the rumen microbiome. Shotgun-sequencing identified the presence of 303 bacterial genera and 171 archaeal species, several of which exhibited differential abundance. Amplicon-sequencing identified 48 bacterial genera, 4 archaeal species, and 9 protozoal species. Among them, 20 bacterial genera and 5 protozoal species were differentially abundant between the two diets. Overall, amplicon-sequencing showed a more drastic diet-derived effect on the ruminal microbial profile compared to shotgun-sequencing. While both methods detected dietary differences at various taxonomic levels, few consistent patterns were evident. Opposite results were seen for the phyla Firmicutes and Bacteroidetes, and the genus
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Registered trials
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