Evidence map›Paper›PMID 34357583›Full record

ArticleBiomolecular NMR assignments2021

Resonance assignment and secondary structure of DbpA protein from the European species, Borrelia afzelii.

Libor Hejduk, Petr Rathner, Martin Strnad, Libor Grubhoffer, Jan Sterba, Ryan O M Rego, Norbert Müller, Adriana Rathner

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecular NMR assignments, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Libor HejdukFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0002-8836-0937
Petr RathnerInstitute of Inorganic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.ORCID 0000-0001-9611-5985
Martin StrnadFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0002-1671-6252
Libor GrubhofferFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0003-0885-1849
Jan SterbaFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0003-1005-1787
Ryan O M RegoFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0001-6932-0940
Norbert MüllerFaculty of Science, University of South Bohemia, Branišovská 1760, 37005, České Budějovice, Czech Republic.ORCID 0000-0002-7621-3980
Adriana RathnerInstitute of Inorganic Chemistry, Johannes Kepler University, Altenbergerstraße 69, 4040, Linz, Austria. adriana.rathner@jku.at.ORCID 0000-0003-2919-9407
University of South Bohemia in České Budějovice · CZJohannes Kepler University of Linz · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Decorin binding proteins (Dbps) mediate attachment of spirochetes in host organisms during the early stages of Lyme disease infection. Previously, different binding mechanisms of Dbps to glycosaminoglycans have been elucidated for the pathogenic species Borrelia burgdorferi sensu stricto and B. afzelii. We are investigating various European Borrelia spirochetes and their interactions at the atomic level using NMR. We report preparative scale recombinant expression of uniformly stable isotope enriched B. afzelii DbpA in Escherichia coli, its chromatographic purification, and solution NMR assignments of its backbone and sidechain

Indexed as

Bacterial ProteinsBorrelia burgdorferi GroupAdhesins, BacterialAmino Acid SequenceEuropeNuclear Magnetic Resonance, BiomolecularProtein Structure, SecondaryAdhesins, BacterialBacterial ProteinsBorrelia afzeliiDecorin-binding proteinsNMR resonance assignment

Identifiers

PMID34357583
PMCPMC8481140
OpenAlexW3192184720

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.