ArticleInsects2021
The USDA-ARS Ag100Pest Initiative: High-Quality Genome Assemblies for Agricultural Pest Arthropod Research.
Article in Insects, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Genomic Structure Predicts Granulovirus Resistance Without a Detectable Life-History Trade-Off in Plodia interpunctella.Molecular ecology · 2026Article
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- Genomic Insights Into the Evolution of Parental Care in Weevils.Genome biology and evolution · 2026Article
- Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).Insect science · 2026Article
- Defining the breakpoints of the vmicroPublication biology · 2026Article
- Self-Assembly ofIndustrial & engineering chemistry research · 2025Article
- Chromosome-level genome assembly of Protandrena (Anthemurgus) passiflorae (Hymenoptera: Andrenidae), a host-plant specialist bee.G3 (Bethesda, Md.) · 2025Article
- Current stewardship practices in invasion biology limit the value and secondary use of genomic data.Molecular ecology resources · 2025Article
- An unusually large genome from an unusually large stonefly: A chromosome-length genome assembly for the giant salmonfly, Pteronarcys californica (Plecoptera: Pteronarcyidae).The Journal of heredity · 2025Article
- Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors.Nature communications · 2025Article
- Exploring the functional properties ofMaterials today. Communications · 2025Article
- Klumpy: A tool to evaluate the integrity of long-read genome assemblies and illusive sequence motifs.Molecular ecology resources · 2025Article
- otb: an automated HiC/HiFi pipeline assembles the Prosapia bicincta Genome.G3 (Bethesda, Md.) · 2024Article
- Reference genome for the Mojave poppy bee (Perdita meconis), a specialist pollinator of conservation concern.The Journal of heredity · 2024Article
- Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes.Nature communications · 2024Article
- Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species.PLoS genetics · 2024Article
- Harvesting Silk Fibers fromACS biomaterials science & engineering · 2024Article
- Direct sequencing of insect symbionts via nanopore adaptive sampling.Current opinion in insect science · 2024Review
- Performance analysis of conventional and AI-based variant callers using short and long reads.BMC bioinformatics · 2023Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phylum Arthropoda includes species crucial for ecosystem stability, soil health, crop production, and others that present obstacles to crop and animal agriculture. The United States Department of Agriculture's Agricultural Research Service initiated the Ag100Pest Initiative to generate reference genome assemblies of arthropods that are (or may become) pests to agricultural production and global food security. We describe the project goals, process, status, and future. The first three years of the project were focused on species selection, specimen collection, and the construction of lab and bioinformatics pipelines for the efficient production of assemblies at scale. Contig-level assemblies of 47 species are presented, all of which were generated from single specimens. Lessons learned and optimizations leading to the current pipeline are discussed. The project name implies a target of 100 species, but the efficiencies gained during the project have supported an expansion of the original goal and a total of 158 species are currently in the pipeline. We anticipate that the processes described in the paper will help other arthropod research groups or other consortia considering genome assembly at scale.
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