ArticleBiomedicines2021
Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study.
Article in Biomedicines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Hydrogel coatings for titanium implants: From smart stimuli-responsive strategies to multimodal bone regeneration.Bioactive materials · 2026Review
- Growth factor-free chondrogenesis and immunomodulation of Wharton's jelly MSCs on chitosan-hyaluronic acid films.Frontiers in bioengineering and biotechnology · 2026Article
- Large-Scale Production of Secretome From Human Dental Pulp Stem Cells for Articular Cartilage Regeneration.Stem cells international · 2026Article
- Review
- Influence of Cell Seeding Density and Material Stiffness on Chondrogenesis of Human Stem Cells Within Soft Hydrogels, Without the Use of Exogenous Growth Factors.Gels (Basel, Switzerland) · 2025Article
- Marine-Derived Polysaccharide Hydrogels as Delivery Platforms for Natural Bioactive Compounds.International journal of molecular sciences · 2025Review
- Cancer Epithelial Cells Participate in the Self-Organization of Lung Tumor Spheroids: A Morphological Approach.Cells, tissues, organs · 2025Article
- Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach.Current issues in molecular biology · 2024Article
- Modulation of 3D Bioprintability in Polysaccharide Bioink by Bioglass Nanoparticles and Multiple Metal Ions for Tissue Engineering.Tissue engineering and regenerative medicine · 2024Article
- Mesenchymal Stem Cells Cultured in a 3D Microgel Environment Containing Platelet-Rich Plasma Significantly Modify Their Chondrogenesis-Related miRNA Expression.International journal of molecular sciences · 2024Article
- Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research.International journal of molecular sciences · 2023Article
- Morphological Characterization of Human Lung Cancer Organoids Cultured in Type I Collagen Hydrogels: A Histological Approach.International journal of molecular sciences · 2023Article
- In Vitro Effect of Δ9-Tetrahydrocannabinol and Cannabidiol on Cancer-Associated Fibroblasts Isolated from Lung Cancer.International journal of molecular sciences · 2022Article
- Alginate: Enhancement Strategies for Advanced Applications.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Matrix-assisted autologous chondrocyte implantation (MACI) has shown promising results for cartilage repair, combining cultured chondrocytes and hydrogels, including alginate. The ability of chondrocytes for MACI is limited by different factors including donor site morbidity, dedifferentiation, limited lifespan or poor proliferation in vitro. Mesenchymal stem cells could represent an alternative for cartilage regeneration. In this study, we propose a MACI scaffold consisting of a mixed alginate-agarose hydrogel in combination with human dental pulp stem cells (hDPSCs), suitable for cartilage regeneration. Scaffolds were characterized according to their rheological properties, and their histomorphometric and molecular biology results. Agarose significantly improved the biomechanical behavior of the alginate scaffolds. Large scaffolds were manufactured, and a homogeneous distribution of cells was observed within them. Although primary chondrocytes showed a greater capacity for chondrogenic differentiation, hDPSCs cultured in the scaffolds formed large aggregates of cells, acquired a rounded morphology and expressed high amounts of type II collagen and aggrecan. Cells cultured in the scaffolds expressed not only chondral matrix-related genes, but also remodeling proteins and chondrocyte differentiation factors. The degree of differentiation of cells was proportional to the number and size of the cell aggregates that were formed in the hydrogels.
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