Evidence map›Paper›PMID 34355202›Full record

ArticleSTAR protocols2021

Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells.

Ishan Agrawal, Nidhi Sharma, Shivanjali Saxena, S Arvind, Debayani Chakraborty, Debarati Bhunia Chakraborty, Deepak Jha, Surajit Ghatak, Sridhar Epari, Tejpal Gupta and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Ishan AgrawalDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Nidhi SharmaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Shivanjali SaxenaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
S ArvindDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Debayani ChakrabortyDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Debarati Bhunia ChakrabortyDepartment of Computer Science and Engineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Deepak JhaDepartment of Neurosurgery, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, India.
Surajit GhatakDepartment of Anatomy, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, India.
Sridhar EpariDepartment of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, India.
Tejpal GuptaDepartment of Radiation Oncology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, India.
Sushmita JhaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
Indian Institute of Technology Jodhpur · INAll India Institute of Medical Sciences Jodhpur · INTata Memorial Hospital · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines. For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).

Indexed as

AnimalsCell LineChromatinDopamineExtracellular TrapsHistonesHumansImmunoassayMiceMicrogliaReactive Oxygen SpeciesChromatinDopamineHistonesReactive Oxygen SpeciesCell-based AssaysImmunologyMicroscopyMolecular BiologyNeuroscience

Identifiers

PMID34355202
PMCPMC8319797
OpenAlexW3187378789

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.