ArticleFrontiers in veterinary science2021
A Combined Flow Cytometric Semen Analysis and miRNA Profiling as a Tool to Discriminate Between High- and Low-Fertility Bulls.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis.Frontiers in veterinary science · 2024Pooled it
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- Current Applications and Future Prospects of High-ThroughputAnimals : an open access journal from MDPI · 2026Review
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Bovine Muscle Satellite Cell-Derived Exosomes Modulate Preadipocyte Adipogenesis via bta-miR-2904.Animals : an open access journal from MDPI · 2026Article
- Application of omics technologies to identify reproductive phenotypes in the context of assisted reproductive technologies for cattle.Animal reproduction · 2026Article
- Deep learning-enabled morphology analysis of bovine sperm for label-free imaging flow cytometry.Frontiers in veterinary science · 2026Article
- Article
- Review
- Sperm Functional Status: A Multiparametric Assessment of the Fertilizing Potential of Bovine Sperm.Veterinary sciences · 2024Article
- Sperm-Borne Mitochondrial Activity Influenced by Season and Age of Holstein Bulls.International journal of molecular sciences · 2024Article
- Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze-Thaw Process.Biopreservation and biobanking · 2024Article
- THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.PloS one · 2024Article
- Effect of cryopreservation and semen extender on extracellular vesicles isolated from bull semen.Frontiers in veterinary science · 2024Article
- Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction.Journal of proteome research · 2023Article
- Transcriptome Profiling of Goose Ovarian Follicle Granulosa Cells Reveals Key Regulatory Networks for Follicle Selection.Animals : an open access journal from MDPI · 2023Article
- CpG DNA methylation changes during epididymal sperm maturation in bulls.Epigenetics & chromatin · 2023Article
- Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders.International journal of molecular sciences · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Investigation of Sperm and Seminal Plasma Candidate MicroRNAs of Bulls with Differing Fertility and In Silico Prediction of miRNA-mRNA Interaction Network of Reproductive Function.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Predicting bull fertility is one of the main challenges for the dairy breeding industry and artificial insemination (AI) centers. Semen evaluation performed in the AI center is not fully reliable to determine the level of bull fertility. Spermatozoa are rich in active miRNA. Specific sperm-borne miRNAs can be linked to fertility. The aim of our study is to propose a combined flow cytometric analysis and miRNA profiling of semen bulls with different fertility to identify markers that can be potentially used for the prediction of field fertility. Sperm functions were analyzed in frozen-thawed semen doses (CG: control group) and high-quality sperm (HQS) fraction collected from bulls with different field fertility levels (estimated relative conception rate or ERCR) by using advanced techniques, such as the computer-assisted semen analysis system, flow cytometry, and small RNA-sequencing. Fertility groups differ for total and progressive motility and in the abnormality degree of the chromatin structure (
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Registered trials
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