Evidence map›Paper›PMID 34354424›Full record

ArticleSaudi journal of biological sciences2021

Proteomic analysis of hypoxia and non-hypoxia secretome mesenchymal stem-like cells from human breastmilk.

Sri Lilidjanti Widjaja, Harsono Salimo, Indah Yulianto, Soetrisno

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Saudi journal of biological sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sri Lilidjanti WidjajaDoctorate Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
Harsono SalimoDepartment of Pediatrics, Dr. Moewardi Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
Indah YuliantoDepartment of Dermatology and Venereology, Dr. Moewardi Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
SoetrisnoDepartment of Obstetrics and Gynecology, Dr. Moewardi Hospital, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
Sebelas Maret University · ID

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionBreastmilk contains proteins and cells which have stem cell properties. The human breastmilk stem cell mimick mesenchymal stem cells and expresses pluripotency genes. The protein level of breastmilk is high in colostrum and gradually subsides in the first year of lactation. The mesenchymal stem cells from breastmilk can be an alternative source of stem cells that can potentially affect cardiovascular therapy. This study aimed to identify the proteomic analysis of secretome mesenchymal stem-like cells under hypoxia compared to non-hypoxia from human breastmilk stem cells. MATERIAL AND

methodsThe human breastmilk was collected from six healthy breastfeeding women and transported to the laboratory under aseptic conditions. The breastmilk cells were isolated then cultured. After 72 h, the human breastmilk stem cells reached confluence then cleaned up and isolated in serum-free media (spheroid) to allow serial passaging every 48 h. The acquisition stem cell was made with flow cytometry. The cells were divided into hBSC secretomes under hypoxia (A) and non-hypoxia (B) and analyzed for LC-MS to identify the peptide structure.

resultsThe human breastmilk cells contained several mesenchymal stem-like cells in density 2.4 × 10

conclusionThe human breastmilk cells contain mesenchymal stem-like cells and a high concentration of CD44, CD73, CD90, and CD105 as surface markers at third passage culture. The hypoxic hBSC secretome produces a higher protein level compare to non-hypoxia. The transforming growth factor -β was found in the hypoxic hBSC secretome as a modulator of VEGF-mediated angiogenesis.

Indexed as

AFP, Alpha-FetoproteinATP, Adenosine TriphosphateBD, Becton DickinsonBMPR-II, Bone morphogenetic protein type IIBSA, Bovine Serum AlbumincDNA, complementary Deoxyribonucleic AcidEHD3, EH Domain-containing Protein 3FACS, Fluorescence-Activated Cell SortingFBS, Fetal Bovine SerumhBSChBSC, Human Breastmilk Stem CellHIF-1α, Hypoxia Inducible Factor-1αHypoxiaIGF1, Insulin-like Growth Factor 1LALBA, α-LactalbuminLC-MSLC-MS, Liquid Chromatography-Mass SpectrometryLF, LactoferrinMAPK, Mitogen-Activated Protein KinaseMesenchymal stem-like cellMPS, Multi Proliferative SupplementMPZL1, Myelin Protein Zero-like Protein 1mRNA, messenger Ribonucleic AcidMSC, Mesenchymal Stem CellPBS, Phosphate-buffered SalineSDS, Sodium Dodecyl SulfateSecretomeSMAD, Signals Mothers Against the DecapentaplegicSMA, Smooth Muscle ActinTGF-β, Transforming Growth Factor-BetaVEGF, Vascular Endothelial Growth Factor

Identifiers

PMID34354424
PMCPMC8324926
OpenAlexW3153865008

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.