ArticleNature methods2021
Ultrasensitive ultrasound imaging of gene expression with signal unmixing.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 83 citations in OpenAlex.
- Trimodal ultrasound imaging of brain-tumor interaction.iScience · 2026Article
- Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.Nature communications · 2026Article
- Sonogenetics for precision medicine: from molecular toolkit to clinical translation.Theranostics · 2026Review
- Ultrasound Imaging Properties of Heterologously Synthesized Gas Vesicles from Halophilic Archaeon.Nanomaterials (Basel, Switzerland) · 2025Article
- A Biomimetic Macrophage-Membrane-Fused Liposomal System Loaded with GVs-HV Recombinant Plasmid for Targeted Anti-Atherosclerosis Therapy.Pharmaceutics · 2025Article
- Multiplexed ultrasound imaging of gene expression.Nature methods · 2025Article
- Enhanced Ultrasound Image Formation With Computationally Efficient Cross-Angular Delay Multiply and Sum Beamforming.Ultrasound in medicine & biology · 2025Article
- Probiotic acoustic biosensors for noninvasive imaging of gut inflammation.Nature communications · 2025Article
- Ultrasound-Mediated Membrane Modulation for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2025Review
- Acoustic percolation switches enable targeted drug delivery controlled by diagnostic ultrasound.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Excavation of acoustic nanostructures biosynthesis gene clusters by combinatorial strategy.Advanced biotechnology · 2025Article
- A mediator-free sonogenetic switch for therapeutic protein expression in mammalian cells.Nucleic acids research · 2025Article
- Ultrafast optical and passive acoustic mapping characterization of nanoscale cavitation nuclei based on gas vesicle proteins.AIP advances · 2025Article
- Genetically engineered gas vesicle proteins with proliferative potential for synergistic targeted tumor therapy.RSC advances · 2025Article
- Achieving single cell acoustic localisation with deactivation super resolution.NPJ acoustics · 2025Article
- Ultrasound Control of Genomic Regulatory Toolboxes for Cancer Immunotherapy.Nature communications · 2024Article
- Harmonic imaging for nonlinear detection of acoustic biomolecules.APL bioengineering · 2024Article
- Elucidating the assembly of gas vesicles by systematic protein-protein interaction analysis.The EMBO journal · 2024Article
- Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening.ACS synthetic biology · 2024Article
- SEMPER: Stoichiometric expression of mRNA polycistrons by eukaryotic ribosomes for compact, ratio-tunable multi-gene expression.Cell systems · 2024Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Acoustic reporter genes (ARGs) that encode air-filled gas vesicles enable ultrasound-based imaging of gene expression in genetically modified bacteria and mammalian cells, facilitating the study of cellular function in deep tissues. Despite the promise of this technology for biological research and potential clinical applications, the sensitivity with which ARG-expressing cells can be visualized is currently limited. Here we present burst ultrasound reconstructed with signal templates (BURST)-an ARG imaging paradigm that improves the cellular detection limit by more than 1,000-fold compared to conventional methods. BURST takes advantage of the unique temporal signal pattern produced by gas vesicles as they collapse under acoustic pressure above a threshold defined by the ARG. By extracting the unique pattern of this signal from total scattering, BURST boosts the sensitivity of ultrasound to image ARG-expressing cells, as demonstrated in vitro and in vivo in the mouse gastrointestinal tract and liver. Furthermore, in dilute cell suspensions, BURST imaging enables the detection of gene expression in individual bacteria and mammalian cells. The resulting abilities of BURST expand the potential use of ultrasound for non-invasive imaging of cellular functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.