ArticleScientific reports2021
Recombinase polymerase amplification (RPA) with lateral flow detection for three Anaplasma species of importance to livestock health.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Molecular prevalence and diagnostic gene targets of Anaplasma, Babesia, Theileria, and Trypanosoma in cattle and buffaloes across Southeast Asia (2015-2025): A systematic review and meta-analysis.Veterinary world · 2026Article
- Development and evaluation of a single-tube RT-ERA-CRISPR/Cas12a assay for simultaneous detection of enterovirus G and recombinant strains with papain-like cysteine protease gene insertion.Veterinary research communications · 2026Article
- Article
- A recombinase polymerase amplification lateral flow assay (RPA-LF) for detection of Babesia divergens-like parasites.Parasites & vectors · 2026Article
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Microscopic and molecular detection ofFrontiers in veterinary science · 2026Article
- Article
- CRISPR-Cas12a-based rapid detection of Babesia gibsoni and Ehrlichia canis in dogs using fluorometer platform.Scientific reports · 2025Article
- Article
- Plasmonic nanoparticle sensors: current progress, challenges, and future prospects.Nanoscale horizons · 2024Review
- RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection.Scientific reports · 2024Article
- Article
- Rapid and Sensitive Detection ofInternational journal of molecular sciences · 2023Article
- Microfluidics-based strategies for molecular diagnostics of infectious diseases.Military Medical Research · 2022Review
- Real-time reverse transcription recombinase polymerase amplification for rapid detection of murine hepatitis virus.Frontiers in microbiology · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anaplasma marginale, A. ovis, and A. phagocytophilum are the causative agents of bovine anaplasmosis, ovine anaplasmosis, and granulocytic anaplasmosis, respectively. The gold standard for diagnosis of post-acute and long-term persistent infections is the serological cELISA, which does not discriminate between Anaplasma species and requires highly equipped laboratories and trained personnel. This study addresses the development of a rapid, isothermal, sensitive, species-specific RPA assays to detect three Anaplasma species in blood and cELISA A. marginale-positive serum samples. Three RPA primer and probe sets were designed targeting msp4 genes of each Anaplasma species and the internal control (GAPDH gene) for each assay. The limit of detection of gel-based or RPA-basic assays is 8.99 × 10
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