ArticleBMC biotechnology2021
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar.
Article in BMC biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 60 citations in OpenAlex.
- Atrazine Degradation by the Nano-Shielded Carbon Dots-Lacassa (CDs-S-M-Lac) System.Nanomaterials (Basel, Switzerland) · 2026Article
- Biomass pyrolysis-derived aqueous phase as a laccase inducer in Pleurotus ostreatus: laccase production, properties, and applications.Bioresources and bioprocessing · 2026Article
- Laccase Immobilization on Carbon-Based Materials Derived from Spent Brewery Grains: Optimization and Stability Evaluation.Molecules (Basel, Switzerland) · 2026Article
- Chemical Modification ofInternational journal of molecular sciences · 2025Article
- Sustainable, Targeted, and Cost-Effective Laccase-Based Bioremediation Technologies for Antibiotic Residues in the Ecosystem: A Comprehensive Review.Biomolecules · 2025Review
- Conductive Hairy Particles With Homogeneous and Janus Design as Carrier Materials for the Efficient Immobilization of Unspecific Peroxygenases.Biotechnology journal · 2025Article
- Enhancing Multi-Enzyme Cascade Activity in Metal-Organic Frameworks via Controlled Enzyme Encapsulation.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Construction and utilization of FeComputational and structural biotechnology journal · 2025Article
- Optimized decolorization of two poly azo dyes Sirius Red and Sirius Blue using laccase-mediator system.3 Biotech · 2024Article
- Structural Insight into the Amino Acid Environment of the Two-Domain Laccase's Trinuclear Copper Cluster.International journal of molecular sciences · 2023Article
- Efficient removal of pharmaceutical contaminants from water and wastewater using immobilized laccase on activated carbon derived from pomegranate peels.Scientific reports · 2023Article
- Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-CNanomaterials (Basel, Switzerland) · 2022Article
- Study of Membrane-Immobilized Oxidoreductases in Wastewater Treatment for Micropollutants Removal.International journal of molecular sciences · 2022Article
- Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review.Frontiers in bioengineering and biotechnology · 2021Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough laccase has a good catalytic oxidation ability, free laccase shows a poor stability. Enzyme immobilization is a common method to improve enzyme stability and endow the enzyme with reusability. Adsorption is the simplest and common method. Modified biochar has attracted great attention due to its excellent performance.
resultsIn this paper, cetyltrimethylammonium bromide (CTAB)-KOH modified biochar (CKMB) was used to immobilize laccase by adsorption method (laccase@CKMB). Based on the results of the single-factor experiments, the optimal loading conditions of laccase@CKMB were studied with the assistance of Design-Expert 12 and response surface methods. The predicted optimal experimental conditions were laccase dosage 1.78 mg/mL, pH 3.1 and 312 K. Under these conditions, the activity recovery of laccase@CKMB was the highest, reaching 61.78%. Then, the CKMB and laccase@CKMB were characterized by TGA, FT-IR, XRD, BET and SEM, and the results showed that laccase could be well immobilized on CKMB, the maximum enzyme loading could reach 57.5 mg/g. Compared to free laccase, the storage and pH stability of laccase@CKMB was improved greatly. The laccase@CKMB retained about 40% of relative activity (4 °C, 30 days) and more than 50% of relative activity at pH 2.0-6.0. In addition, the laccase@CKMB indicated the reusability up to 6 reaction cycles while retaining 45.1% of relative activity. Moreover, the thermal deactivation kinetic studies of laccase@CKMB showed a lower k value (0.00275 min
conclusionsWe explored scientific and reasonable immobilization conditions of laccase@CKMB, and the laccase@CKMB possessed relatively better stabilities, which gave the immobilization of laccase on this cheap and easily available carrier material the possibility of industrial applications.
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