ArticleProtein and peptide letters2021
Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.
Article in Protein and peptide letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- A conserved dimerization element is required for protein kinase activation bybioRxiv : the preprint server for biology · 2026Article
- Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.Microbial cell factories · 2026Article
- Statistical approach for media optimization of fHbp-PorA chimeric protein expression in Escherichia coli; development of a promising Meningococcal B vaccine.Journal of industrial microbiology & biotechnology · 2026Article
- High-Throughput Pipeline for Protein Expression and Solubility Profiling Using Synthetically Generated Plasmids.Current protocols · 2025Article
- Foot-and-Mouth Disease Virus-like Particles Produced inVeterinary sciences · 2025Article
- Enhancing recombinant growth factor and serum protein production for cultivated meat manufacturing.Microbial cell factories · 2025Review
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Expression of soluble moloney murine leukemia virus-reverse transcriptase in Escherichia coli BL21 star (DE3) using autoinduction system.Molecular biology reports · 2024Article
- Factors involved in heterologous expression of proteins in E. coli host.Archives of microbiology · 2023Review
- Review
- Construction of an AI-2 quorum sensing induced heterologous protein expression system inPeerJ · 2021Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAuto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead to variations in the induction level.
objectivesIn this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.
methodsFirst, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter. Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) and Thermus thermophilus manganese-catalase (T7 lac promoter-based expression).
resultsAuto-induced GFPuv expression was observed with the enzymatic protein digest Polypepton, but not with another digest tryptone. Regardless of the type of protein digest, supplementing Terrific Broth medium with glucose (at a final concentration of 2.9 g/L) and lactose (at a final concentration of 7.6 g/L) was successful in obtaining an induction level similar to that achieved with a commercially available auto-induction medium. The two recombinant proteins were produced in milligram quantity of purified protein per liter of culture.
conclusionThe medium composition shown in this study would be practically useful for attaining reliable auto-induction for E. coli-based recombinant protein production.
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