ArticleEnvironmental research2022
Discrimination of non-infectious SARS-CoV-2 particles from fomites by viability RT-qPCR.
Article in Environmental research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Comparative evaluation of viability PCR reagents highlights the superior efficacy of PMAxx azo dye for bacterial and viral viability discrimination using real-time and digital PCR.Microbiology spectrum · 2026Article
- Pathogens on High-Touch Surfaces in an Arid Megacity: A Longitudinal Molecular Surveillance Study.Microorganisms · 2026Article
- Methods for detecting highly pathogenic avian influenza H5N1 virus in dairy processing environments.Frontiers in microbiology · 2026Article
- From Surfaces to Spillover: Environmental Persistence and Indirect Transmission of Influenza A(H3N8) Virus.Microorganisms · 2025Article
- Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification.Virologica Sinica · 2025Article
- Antiviral Effects of Pyrroloquinoline Quinone through Redox Catalysis To Prevent Coronavirus Infection.ACS omega · 2023Article
- A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment.Veterinary sciences · 2023Article
- Porcine Epidemic Diarrhea Virus, Surrogate for Coronavirus Decay Measurement in French Coastal Waters and Contribution to Coronavirus Risk Evaluation.Microbiology spectrum · 2023Article
- Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil.Viruses · 2023Article
- Evaluating Whether and How Public Health Event Information Frameworks Promote Pro-Environmental Behavior.International journal of environmental research and public health · 2023Article
- Cytomegalovirus in children undergoing haematopoietic stem cell transplantation: a diagnostic and therapeutic approach to antiviral resistance.Frontiers in pediatrics · 2023Review
- Transmission and control of SARS-CoV-2 on ground public transport: A rapid review of the literature up to May 2021.Journal of transport & health · 2022Review
- Platinum chloride-based viability RT-qPCR for SARS-CoV-2 detection in complex samples.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ongoing coronavirus 2019 (COVID-19) pandemic constitutes a concerning global threat to public health and economy. In the midst of this pandemic scenario, the role of environment-to-human COVID-19 spread is still a matter of debate because mixed results have been reported concerning SARS-CoV-2 stability on high-touch surfaces in real-life scenarios. Up to now, no alternative and accessible procedures for cell culture have been applied to evaluate SARS-CoV-2 infectivity on fomites. Several strategies based on viral capsid integrity have latterly been developed using viability markers to selectively remove false-positive qPCR signals resulting from free nucleic acids and damaged viruses. These have finally allowed an estimation of viral infectivity. The present study aims to provide a rapid molecular-based protocol for detection and quantification of viable SARS-CoV-2 from fomites based on the discrimination of non-infectious SARS-CoV-2 particles by platinum chloride (IV) (PtCl
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Registered trials
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