Evidence map›Paper›PMID 34351778›Full record

ArticleJournal of proteome research2021

Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells.

Kendall Martin, Tong Zhang, Tai-Tu Lin, Amber N Habowski, Rui Zhao, Chia-Feng Tsai, William B Chrisler, Ryan L Sontag, Daniel J Orton, Yong-Jie Lu and 8 more

Open access · greenAbstract read
In one paragraph

Article in Journal of proteome research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 2 countries.

Kendall MartinBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Tong ZhangBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Tai-Tu LinBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Amber N HabowskiDepartment of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California 92697, United States.
Rui ZhaoEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Chia-Feng TsaiBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0002-6514-6911
William B ChrislerBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Ryan L SontagBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Daniel J OrtonBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Yong-Jie LuCentre for Cancer Biomarker and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, United Kingdom.
Karin D RodlandBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0001-7070-6541
Bin YangBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0003-1686-8800
Tao LiuBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0001-9529-6550
Richard D SmithBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0002-2381-2349
Wei-Jun QianBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0002-5393-2827
Marian L WatermanDepartment of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California 92697, United States.
H Steven WileyEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Tujin ShiBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.ORCID 0000-0002-5592-3588
Pacific Northwest National Laboratory · USUniversity of California, Irvine · USQueen Mary University of London · GB

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Christine Hui · 1994 to 2026
$57.9M
Equipment Supplement Request for a Research Resource for Ultra-sensitive High Throughput ProteomicsP41GM103493 · NIGMS · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI SMITH, RICHARD D · 2012 to 2022
$22.6M
TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI MACCOSS, MICHAEL · 2012 to 2021
$20.7M
Project 4: Novel reagent development to enable molecular characterizationU19AG065156 · NIA · UNIVERSITY OF WASHINGTON · PI MACCOSS, MICHAEL, MONTINE, THOMAS J · 2020 to 2024
$15.9M
Shared Resource Core: Single Cell AnalysisU54CA217378 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2018 to 2022
$9.7M
CARCINOGENESIST32CA009054 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI EDINGER, AIMEE L, FRUMAN, DAVID ALEXANDER · 1985 to 2025
$8.6M
PNNL Proteome Characterization CenterU24CA210955 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI LIU, TAO, SMITH, RICHARD D · 2016 to 2020
$5.3M
Reverse Sensitivity Analysis for Identifying Predictive Proteomics Signatures of CancerU01CA227544 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI QIAN, WEI-JUN, SAURO, HERBERT M. · 2019 to 2023
$3.1M
Quantifying proteins in plasma to democratize personalized medicine for patients with type 1 diabetesU01DK121289 · NIDDK · UNIVERSITY OF WASHINGTON · PI HOOFNAGLE, ANDREW N · 2019 to 2021
$1.9M
A streamlined platform for phosphoproteome mapping of human tissuesUH3CA256967 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI LIU, HUIPING, SHI, TUJIN · 2022 to 2023
$1.2M
A streamlined platform for phosphoproteome mapping of human tissuesUG3CA256967 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI LIU, HUIPING, SHI, TUJIN · 2020 to 2021
$800k
An ultrasensitive targeted mass spectrometry system for proteomics analysis of single cellsR21CA223715 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI SHI, TUJIN · 2018 to 2020
$691k
NCI NIH HHS P30 CA062203NCI NIH HHS R21 CA223715NCI NIH HHS T32 CA009054NCI NIH HHS U01 CA227544NCI NIH HHS U24 CA210955NCI NIH HHS U54 CA217378NCI NIH HHS UG3 CA256967NCI NIH HHS UH3 CA256967NIA NIH HHS U19 AG065156NIDDK NIH HHS U01 DK121289NIGMS NIH HHS P41 GM103493NIGMS NIH HHS P41 GM103533
6 · The paper itself

Abstract

Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (

Indexed as

ProteomeProteomicsAnimalsChromatography, LiquidGene Expression ProfilingMass SpectrometryMiceProteomecolon crypt cellsnanoproteomicssmall numbers of cellsSOPs-MSsurfactant DDM

Identifiers

PMID34351778
PMCPMC8945255
OpenAlexW3190820600

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.