ArticleMolecular biology reports2021
CRISPR/Cas9-mediated mutagenesis of WRKY3 and WRKY4 function decreases salt and Me-JA stress tolerance in Arabidopsis thaliana.
Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 69 citations in OpenAlex.
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- WRKY transcription factors govern selenium metabolism in Broussonetia papyrifera: genome-wide identification, expression profiling, and regulatory network analysis.BMC plant biology · 2026Article
- Genome-wide analysis of DC1 domain proteins inFrontiers in plant science · 2026Article
- TaIRE1-mediated unconventional splicing of the TabZIP60 mRNA and the miR172 precursor regulates heat stress tolerance in wheat.Journal of integrative plant biology · 2025Article
- Ancient allopolyploidy and specific subgenomic evolution drove the radiation of poplars and willows.Nature communications · 2025Article
- CRISPR/Cas9-mediated editing of jasmonic acid pathways to enhance biotic & abiotic stress tolerance: An overview & prospects.Functional & integrative genomics · 2025Review
- Physiological and molecular mechanisms of nitrogen in alleviating drought stress in Phoebe bournei.Scientific reports · 2025Article
- Roles of WRKY Transcription Factors in Response to Sri Lankan Cassava Mosaic Virus Infection in Susceptible and Tolerant Cassava Cultivars.Plants (Basel, Switzerland) · 2025Article
- The role of DNA content in shaping chromatin architecture and gene expression.The Plant journal : for cell and molecular biology · 2025Article
- Identification and expression analysis of the RBOH gene family of Isatis indigotica Fort. and the potential regulation mechanism of RBOH gene on HPlant cell reports · 2025Article
- Ploidy levels influence cold tolerance of Cyclocarya paliurus: insight into the roles of WRKY genes.BMC genomics · 2025Article
- Genome-wide identiffcation of the WRKY gene family in poplar and the positive role of PsnWRKY95 in response to cadmium stress.PloS one · 2025Article
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- CRISPR-Cas9 mediated understanding of plants' abiotic stress-responsive genes to combat changing climatic patterns.Functional & integrative genomics · 2024Review
- Comprehensive Expression Analysis of the WRKY Gene Family inInternational journal of molecular sciences · 2024Article
- A review on strategies for crop improvement against drought stress through molecular insights.3 Biotech · 2024Review
- Identification of genetic loci and candidate genes regulating photosynthesis and leaf morphology through genome-wide association study inFrontiers in plant science · 2024Article
- CRISPR/Cas9-gene editing approaches in plant breeding.GM crops & food · 2023Review
- Gene Expression and Interaction Analysis ofPlants (Basel, Switzerland) · 2023Article
- Allelic variation in transcription factor PtoWRKY68 contributes to drought tolerance in Populus.Plant physiology · 2023Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundWRKY transcription factor is involved in regulation of plant growth and development, response to biotic and abiotic stresses, including homologous WRKY3 and WRKY4 genes which play a vital role in regulating plants defense response to pathogen and drought stress. METHODS AND
resultsTo investigate the function of AtWRKY3 and AtWRKY4 genes in regulating salt and Me-JA stresses, the loss-of-function mutations were generated by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) system in Arabidopsis thaliana. Several independent transgenic lines with single or double mutations were obtained via Agrobacterium-mediated transformation. The knockout lines of AtWRKY3 and AtWRKY4 genes were successfully achieved and confirmed by qRT-PCR technology. Expression analysis showed that AtWRKY3 and AtWRKY4 genes had significantly up-regulated under salt and Me-JA stresses. The growth of double mutant plants under salt or Me-JA stresses were significantly inhibited compared with corresponding wild type (WT) plants, especially their root lengths. Moreover, the double mutant plants displayed salt and Me-JA sensitivity phenotypic characteristics, such as the increased relative electrolyte leakage (REL) and a substantial reduce in the activities of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities.
conclusionTaken together, these data suggested that the simultaneous modification of homologous gene copies of WRKY are established using CRISPR/Cas9 system in A. thaliana and the loss of AtWRKY3 and AtWRKY4 has an effect on ROS scavenging pathways to reduce stress tolerance.
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