Evidence map›Paper›PMID 34350538›Full record

ArticleJournal of endocrinological investigation2022

Pregnancy-associated plasma protein A mRNA expression as a marker for differentiated thyroid cancer: results from a "surgical" and a "cytological" series.

C Marzocchi, M Capezzone, A Sagnella, A Cartocci, M Caroli Costantini, L Brindisi, V Mancini, S Cantara, M G Castagna

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In one paragraph

Article in Journal of endocrinological investigation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. mCell biology and toxicology · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

C MarzocchiDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.ORCID http://orcid.org/0000-0002-8573-4913
M CapezzoneDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.ORCID http://orcid.org/0000-0001-8624-5831
A SagnellaDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.ORCID http://orcid.org/0000-0003-2247-9027
A CartocciDepartment of Medical Biotechnologies, University of Siena, Siena, Italy.ORCID http://orcid.org/0000-0002-1818-6275
M Caroli CostantiniDepartment of Oncology and Pathological Anatomy, Azienda Ospedaliera, Universitario Senese, Siena, Italy.ORCID http://orcid.org/0000-0001-7803-6060
L BrindisiDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.ORCID http://orcid.org/0000-0003-2422-2575
V ManciniDepartment of Medical Biotechnologies, University of Siena, Siena, Italy.ORCID http://orcid.org/0000-0002-4775-3777
S Cantara *Department of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy. cantara@unisi.it.ORCID http://orcid.org/0000-0002-5741-295X
M G Castagna *Department of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.ORCID http://orcid.org/0000-0002-2457-1751

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposePregnancy-associated plasma protein A (PAPPA) is a metalloproteinase initially described for its role during pregnancy. PAPPA regulates IGF ligands 1 (IGF1) bioavailability through the degradation of IGF-binding protein 4 (IGFBP4). After the cleavage of IGFBP4, free IGF1 is able to bind IGF1 receptors (IGF1R) triggering the downstream signaling. Recently, PAPPA expression has been linked with development of several cancers. No data have been published on thyroid cancer, yet.

methodsWe evaluated PAPPA, insulin-like growth factor (IGF1), IGF1 receptors (IGF1R) and IGF-binding protein 4 (IGFBP4) mRNA expression levels in a "Surgical series" of 94 thyroid nodules (64 cancers, 16 follicular adenomas and 14 hyperplastic nodules) and in a "Cytological series" of 80 nodules from 74 patients underwent to fine-needle aspiration cytology (FNAC). In tissues, PAPPA was also evaluated by western blot.

resultsWe found that PAPPA expression was increased in thyroid cancer specimen at mRNA and protein levels and that, adenomas and hyperplastic nodules had an expression similar to normal tissues. When applied on thyroid cytologies, PAPPA expression was able to discriminate benign from malignant nodules contributing to pre-surgical classification of the nodules. We calculated a cut-off with a good specificity (91%) which reached 100% when combined with molecular biology.

conclusionThese results show that PAPPA could represent a promising diagnostic marker for differentiated thyroid cancer.

Indexed as

Pregnancy-Associated Plasma Protein-AThyroid GlandThyroid NeoplasmsBiomarkers, TumorBiopsy, Fine-NeedleFemaleGene Expression ProfilingHumansInsulin-Like Growth Factor Binding Protein 4Insulin-Like Growth Factor IMaleMiddle AgedReceptor, IGF Type 1RNA, MessengerSensitivity and SpecificitySignal TransductionBiomarkers, TumorIGF1 protein, humanIGFBP4 protein, humanInsulin-Like Growth Factor Binding Protein 4Insulin-Like Growth Factor IPAPPA protein, humanPregnancy-Associated Plasma Protein-AReceptor, IGF Type 1RNA, MessengerFNACIGF pathwayPregnancy-associated plasma protein AThyroid cancerTumor marker

Identifiers

PMID34350538
PMCPMC8783868

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.