ArticleFrontiers in veterinary science2021
Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Sperm RNA landscape during sexual maturation in Duroc boars.BMC genomics · 2026Article
- Transcriptome analysis to identify candidate genes related to mammary gland development of Bactrian camel (Frontiers in veterinary science · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by TargetingFrontiers in cell and developmental biology · 2021Article
- Significance and Relevance of Spermatozoal RNAs to Male Fertility in Livestock.Frontiers in genetics · 2021Review
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Authors and funding
7 authors.
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Abstract
RNA-Seq data from human semen suggests that the study of the sperm transcriptome requires the previous elimination from the ejaculates of somatic cells carrying a larger load of RNA. Semen purification is also carried to study the sperm transcriptome in other species including swine and it is often done by density gradient centrifugation to obtain viable spermatozoa from fresh ejaculates or artificial insemination doses, thereby limiting the throughput and remoteness of the samples that can be processed in one study. The aim of this work was to evaluate the impact of purification with density gradient centrifugation by BoviPure
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