ArticleLiver research (Beijing, China)2021
A fluorescence imaging based-assay to monitor mitophagy in cultured hepatocytes and mouse liver.
Article in Liver research (Beijing, China), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Hepatic STEAP4 promotes liver regeneration by regulating lysosomal iron homeostasis and membrane integrity in acetaminophen-induced liver injury.Hepatology (Baltimore, Md.) · 2026Article
- Development and characterization of experimental β-cell senescence models revealing autophagy defects and altered stimulus-secretion coupling.GeroScience · 2026Article
- BVDV utilizes PGC-1α downregulation to remodel the mitochondrial metabolic microenvironment, enhancing viral replication and impairing host immunity.Frontiers in veterinary science · 2026Article
- A unified intracellular pH landscape with SITE-pHorin: a quantum-entanglement-enhanced pH probe.Science China. Life sciences · 2026Article
- Rac1 promotes proximal tubule kidney repair by coupling the actin cytoskeleton to mitochondrial function.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Amyloid-β protein precursor modulates mitophagy and mitochondrial function through its cellular localization.Journal of Alzheimer's disease : JAD · 2025Article
- Mitochondrial and microtubule defects in Exfoliation Glaucoma.Free radical biology & medicine · 2025Article
- Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection.PLoS pathogens · 2025Article
- Sox17 protects human brain microvascular endothelial cells from AngII-induced injury by regulating autophagy and apoptosis.Molecular and cellular biochemistry · 2024Article
- The actin binding protein profilin 1 localizes inside mitochondria and is critical for their function.EMBO reports · 2024Article
- A spatial map of hepatic mitochondria uncovers functional heterogeneity shaped by nutrient-sensing signaling.Nature communications · 2024Article
- Monitoring Mitophagy Dynamics in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- ACSS2 gene variants determine kidney disease risk by controlling de novo lipogenesis in kidney tubules.The Journal of clinical investigation · 2023Article
- Mitochondria-lysosome-related organelles mediate mitochondrial clearance during cellular dedifferentiation.Cell reports · 2023Article
- Article
- The actin binding protein profilin 1 is critical for mitochondria function.bioRxiv : the preprint server for biology · 2023Article
- Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells.Journal of visualized experiments : JoVE · 2023Article
- Cell type and sex specific mitochondrial phenotypes in iPSC derived models of Alzheimer's disease.Frontiers in molecular neuroscience · 2023Article
- Acute exercise rapidly activates hepatic mitophagic flux.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Article
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2 authors at 1 institution in 1 country.
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Abstract
BACKGROUND AND
aimMitophagy is a lysosomal degradation pathway that selectively removes damaged, aged and dysfunctional mitochondria. Recent advances in understanding mitophagy highlight its importance in various physiological and pathological conditions including liver diseases. However, reliable quantitative assays to monitor mitophagy in cultured cells and in tissues are still scarce.
methodsWe describe a detailed protocol for monitoring mitophagy in primary cultured hepatocytes and mouse livers using cytochrome C oxidase subunit 8 (Cox8)-enhanced green fluorescent protein (EGFP)-mCherry, a dual color fluorescence based-imaging method.
resultsMitochondria are visualized in yellow fluorescence due to the merged EGFP and mCherry signal. In contrast, autolysosome enclosed mitochondria are shown as red puncta due to quenching of EGFP green fluorescence in acidic compartments. Quantifying the number of red-only puncta in each cell can obtain a quantitative measure for mitophagy.
conclusionsCox8-EGFP-mCherry assay can specifically target to mitochondria and be used to monitor mitophagy
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