Evidence map›Paper›PMID 34349113›Full record

ArticleNature communications2021

Puf6 primes 60S pre-ribosome nuclear export at low temperature.

Stefan Gerhardy, Michaela Oborská-Oplová, Ludovic Gillet, Richard Börner, Rob van Nues, Alexander Leitner, Erich Michel, Janusz J Petkowski, Sander Granneman, Roland K O Sigel and 2 more

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. FRET-guided selection of RNA 3D structures.Nucleic acids research · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Diversity and structural-functional insights of alpha-solenoid proteins.Protein science : a publication of the Protein Society · 2024
    Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 4 countries.

Stefan Gerhardy *Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
Michaela Oborská-Oplová *Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-0976-4341
Ludovic GilletInstitute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-1001-3265
Richard BörnerDepartment of Chemistry, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0001-8407-6624
Rob van NuesCentre for Synthetic and Systems Biology (SynthSys), University of Edinburgh, Edinburgh, UK.ORCID http://orcid.org/0000-0003-0325-1481
Alexander LeitnerInstitute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0003-4126-0725
Erich MichelDepartment of Biochemistry, University of Zurich, Zurich, Switzerland.
Janusz J PetkowskiInstitute of Biochemistry, ETH Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-1921-4848
Sander GrannemanCentre for Synthetic and Systems Biology (SynthSys), University of Edinburgh, Edinburgh, UK.
Roland K O SigelDepartment of Chemistry, University of Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-1307-7993
Ruedi AebersoldInstitute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-9576-3267
Vikram Govind PanseInstitute of Medical Microbiology, University of Zurich, Zurich, Switzerland. vpanse@imm.uzh.ch.ORCID http://orcid.org/0000-0002-4770-7068
University of Zurich · CHETH Zurich · CHUniversity of Edinburgh · GBHochschule Mittweida · DE

Funding

Medical Research Council MR/R008205/1Wellcome TrustWellcome Trust 091549
6 · The paper itself

Abstract

Productive ribosomal RNA (rRNA) compaction during ribosome assembly necessitates establishing correct tertiary contacts between distant secondary structure elements. Here, we quantify the response of the yeast proteome to low temperature (LT), a condition where aberrant mis-paired RNA folding intermediates accumulate. We show that, at LT, yeast cells globally boost production of their ribosome assembly machinery. We find that the LT-induced assembly factor, Puf6, binds to the nascent catalytic RNA-rich subunit interface within the 60S pre-ribosome, at a site that eventually loads the nuclear export apparatus. Ensemble Förster resonance energy transfer studies show that Puf6 mimics the role of Mg

Indexed as

Active Transport, Cell NucleusCell NucleusCold TemperatureGTP PhosphohydrolasesMutationProtein BindingProtein Interaction Domains and MotifsProteomeRibosomesRibosome Subunits, Large, EukaryoticRNA-Binding ProteinsRNA FoldingRNA PrecursorsRNA, RibosomalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsGTP PhosphohydrolasesNOG2 protein, S cerevisiaeProteomePuf6 protein, S cerevisiaeRNA-Binding ProteinsRNA PrecursorsRNA, RibosomalSaccharomyces cerevisiae Proteins

Identifiers

PMID34349113
PMCPMC8338941
OpenAlexW3187722524

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.