ArticleApplied and environmental microbiology2021
Glutathione Synthetase Overexpression in Acidithiobacillus ferrooxidans Improves Halotolerance of Iron Oxidation.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Identification and characterization of quorum-quenching enzyme AiiF fromApplied and environmental microbiology · 2026Article
- Towards Sustainable Bioleaching of Platinum Group Metals from Spent Automotive Catalysts.Materials (Basel, Switzerland) · 2026Review
- Ferroptosis bridges early-life oxidative stress and lifetime meat quality defects in broilers.Poultry science · 2026Article
- Reduced glutathione levels inmSystems · 2026Article
- The outer membrane inApplied and environmental microbiology · 2025Article
- Proteomic Insights into the Adaptation ofJournal of proteome research · 2025Article
- Overexpression of sulfide:quinone reductase (SQR) inApplied and environmental microbiology · 2025Article
- Study on the intracellular adaptative mechanism of Acidithiobacillus caldus MTH-04 to NaCl stress.Microbial cell factories · 2023Article
- Overexpression of quorum sensing genes inBiotechnology reports (Amsterdam, Netherlands) · 2023Article
- Advances in bioleaching of waste lithium batteries under metal ion stress.Bioresources and bioprocessing · 2023Review
- Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation - part A.Applied microbiology and biotechnology · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidithiobacillus ferrooxidans is a well-studied iron- and sulfur-oxidizing acidophilic chemolithoautotroph that is exploited for its ability to participate in the bioleaching of metal sulfides. Here, we overexpressed the endogenous glutamate-cysteine ligase and glutathione synthetase genes in separate strains and found that glutathione synthetase overexpression increased intracellular glutathione levels. We explored the impact of pH on the halotolerance of iron oxidation in wild-type and engineered cultures. The increase in glutathione allowed the modified cells to grow under salt concentrations and pH conditions that are fully inhibitory to wild-type cells. Furthermore, we found that improved iron oxidation ability in the presence of chloride also resulted in higher levels of intracellular reactive oxygen species (ROS) in the strain. These results indicate that glutathione overexpression can be used to increase halotolerance in A. ferrooxidans and would likely be a useful strategy on other acidophilic bacteria.
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