ReviewJournal of biosciences2021
Excitation light-induced phototoxicity during fluorescence imaging.
Review in Journal of biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Investigation of Viscoelastic Properties of Macrophage Membrane-Cytoskeleton Induced by Gold Nanorods in Leishmania Infection.Nanomaterials (Basel, Switzerland) · 2025Article
- Research Progress of Persistent Luminescence Nanoparticles in Biological Detection Imaging and Medical Treatment.Materials (Basel, Switzerland) · 2025Review
- Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.Plant cell reports · 2025Article
- Evaluation of image analysis tools for the measurement of cellular morphology.Frontiers in cell and developmental biology · 2025Review
- Vertical Microfluidic Trapping System for Capturing and Simultaneous Electrochemical Detection of Cells.Sensors (Basel, Switzerland) · 2024Article
- Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization.View (Beijing, China) · 2024Article
- An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non-Excitable Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Photobleaching and phototoxicity of mitochondria in live cell fluorescent super-resolution microscopy.Mitochondrial communications · 2024Article
- Review
- Optical Activation of Photoswitchable TRPC Ligands in the Mammalian Olfactory System Using Laser Scanning Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Is Drp1 sufficient to catalyze membrane fission?Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy has revolutionized life science research. It is a powerful technique to visualize molecular structures, physiological functions, and dynamic processes in living cells, tissues, and organisms. Excitation-light and/or excited-fluorescent proteins-mediated dysregulation in cell physiology are widely known but poorly considered. Although there are vast applications of fluorescence microscopy in research, imaging results may suffer from the excitation-light-induced artefacts. Here, we highlight potential excitation light-induced alterations in cell functions thereby misinterpretation of imaging results and strategies to minimize common artefacts that can be produced during imaging experiments.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.