ArticleReproductive biomedicine online2021
Assessing the testicular sperm microbiome: a low-biomass site with abundant contamination.
Article in Reproductive biomedicine online, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Maternal and infant gut microbiome.iMeta · 2026Review
- Unfolding the Semen Microbiota: Implications for Male Infertility.Biomedicines · 2026Review
- Probiotics as Microbiome Modulators in Male Infertility: Rethinking Dysbiosis Across the Gut-Testis Axis.Journal of personalized medicine · 2026Review
- Seminal dysbiosis in idiopathic male infertility: toward a seminal dysbiosis-inflammatory/oxidative endophenotype-SDF axis.Frontiers in endocrinology · 2026Review
- Transgenerational exposure to plastics-derived endocrine-disrupting bisphenol A and its analogs on male infertility: impact of gut dysbiosis and epigenetic regulation.Frontiers in endocrinology · 2026Review
- Single-cell analysis of testicular bacterial microbiome changes during aging and effect on reproductive capacity in mice.iScience · 2025Article
- Review
- Article
- Lifestyle and Environmental Factors Affecting Male Fertility, Individual Predisposition, Prevention, and Intervention.International journal of molecular sciences · 2025Review
- Puberty status influences bacterial communities of the boar urogenital tract.Journal of animal science · 2025Article
- State-of-the-art approaches in the investigation of human seminal bacteriome using metagenomic methods.Frontiers in reproductive health · 2025Review
- Article
- Dipeptide metabolite, glutamyl-glutamate mediates microbe-host interaction to boost spermatogenesis.Scientific reports · 2024Article
- Male Tract Microbiota and Male Infertility.Cells · 2024Review
- The microbiota continuum along the upper reproductive tract of male rat and its relation to semen parameters.Heliyon · 2024Article
- Effect of environmental factors on seminal microbiome and impact on sperm quality.Frontiers in endocrinology · 2024Review
- Gut microbiota-gonadal axis: the impact of gut microbiota on reproductive functions.Frontiers in immunology · 2024Review
- Exploring the microbiome-gut-testis axis in testicular germ cell tumors.Frontiers in cellular and infection microbiology · 2024Review
- Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders.Medicina (Kaunas, Lithuania) · 2023Review
- Human Male Genital Tract Microbiota.International journal of molecular sciences · 2023Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
research questionThe semen harbours a diverse range of microorganisms. The origin of the seminal microbes, however, has not yet been established. Do testicular spermatozoa harbour microbes and could they potentially contribute to the seminal microbiome composition?
designThe study included 24 samples, comprising a total of 307 testicular maturing spermatozoa. A high-throughput sequencing method targeting V3 and V4 regions of 16S rRNA gene was applied. A series of negative controls together with stringent in-silico decontamination methods were analysed.
resultsBetween 50 and 70% of all the detected bacterial reads accounted for contamination in the testicular sperm samples. After stringent decontamination, Blautia (P = 0.04), Cellulosibacter (P = 0.02), Clostridium XIVa (P = 0.01), Clostridium XIVb (P = 0.04), Clostridium XVIII (P = 0.02), Collinsella (P = 0.005), Prevotella (P = 0.04), Prolixibacter (P = 0.02), Robinsoniella (P = 0.04), and Wandonia (P = 0.04) genera demonstrated statistically significant abundance among immature spermatozoa.
conclusionsOur results indicate that the human testicle harbours potential bacterial signature, though in a low-biomass, and could contribute to the seminal microbiome composition. Further, applying stringent decontamination methods is crucial for analysing microbiome in low-biomass site.
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