ArticleMicrobial cell factories2021
Vanillic acid and methoxyhydroquinone production from guaiacyl units and related aromatic compounds using Aspergillus niger cell factories.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Cholesterol-lowering effects of oats induced by microbially produced phenolic metabolites in metabolic syndrome: a randomized controlled trial.Nature communications · 2026Trial
- Targeted Metabolomic Profiling of Emotional and Reflex Tears: A Paired Exploratory Study.Metabolites · 2026Article
- The oleaginous yeast Cutaneotrichosporon oleaginosum modifies corn stover alkali lignin.Scientific reports · 2026Article
- FAMeDB: A Curated Database for the Analysis of Fungal Aromatic Compound Metabolism.Computational and structural biotechnology journal · 2026Article
- Cross-kingdom comparative genomics reveal the metabolic potential of fungi for lignin turnover in deadwood.Nature ecology & evolution · 2025Article
- Transcriptome analysis of Aspergillus oryzae RIB40 under chemical stress reveals mechanisms of adaptation to fungistatic compounds of lignocellulosic side streams.Biotechnology for biofuels and bioproducts · 2025Article
- An Updated Perspective on the Aromatic Metabolic Pathways of Plant-Derived Homocyclic Aromatic Compounds inMicroorganisms · 2025Review
- Time-Series Metabolome and Transcriptome Analyses Reveal the Genetic Basis of Vanillin Biosynthesis in Vanilla.Plants (Basel, Switzerland) · 2025Article
- Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.Bioprocess and biosystems engineering · 2025Article
- Valorization of okra waste: Microencapsulation of okra flower polyphenol-rich extract with maltodextrin and gum Arabic by freeze drying, spray drying, and microwave drying.Journal of food science · 2025Article
- Bioconversion of Food and Green Waste into Valuable Compounds Using Solid-State Fermentation in Nonsterile Conditions.Plants (Basel, Switzerland) · 2024Article
- Filamentous fungi as emerging cell factories for the production of aromatic compounds.Fungal biology and biotechnology · 2024Review
- The Application of Untargeted Metabolomic Approaches for the Search of Common Bioavailable Metabolites in Human Plasma Samples fromJournal of agricultural and food chemistry · 2024Article
- Investigation of the phytochemical profiling and antioxidant, anti-diabetic, anti-inflammatory, and MDA-MB-231 cell line antiproliferative potentials of extracts from Ephedra alata Decne.Scientific reports · 2024Article
- The shikimate pathway: gateway to metabolic diversity.Natural product reports · 2024Review
- Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review.Biomolecules · 2023Review
- Degradation of Lignin by Infrared Free Electron Laser.Polymers · 2022Article
- Low-Temperature Biodegradation of Lignin-Derived Aromatic Model Monomers by the Cold-Adapted YeastMicroorganisms · 2022Article
- Utilization of ferulic acid inFrontiers in fungal biology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe aromatic compounds vanillin and vanillic acid are important fragrances used in the food, beverage, cosmetic and pharmaceutical industries. Currently, most aromatic compounds used in products are chemically synthesized, while only a small percentage is extracted from natural sources. The metabolism of vanillin and vanillic acid has been studied for decades in microorganisms and many studies have been conducted that showed that both can be produced from ferulic acid using bacteria. In contrast, the degradation of vanillin and vanillic acid by fungi is poorly studied and no genes involved in this metabolic pathway have been identified. In this study, we aimed to clarify this metabolic pathway in Aspergillus niger and identify the genes involved.
resultsUsing whole-genome transcriptome data, four genes involved in vanillin and vanillic acid metabolism were identified. These include vanillin dehydrogenase (vdhA), vanillic acid hydroxylase (vhyA), and two genes encoding novel enzymes, which function as methoxyhydroquinone 1,2-dioxygenase (mhdA) and 4-oxo-monomethyl adipate esterase (omeA). Deletion of these genes in A. niger confirmed their role in aromatic metabolism and the enzymatic activities of these enzymes were verified. In addition, we demonstrated that mhdA and vhyA deletion mutants can be used as fungal cell factories for the accumulation of vanillic acid and methoxyhydroquinone from guaiacyl lignin units and related aromatic compounds.
conclusionsThis study provides new insights into the fungal aromatic metabolic pathways involved in the degradation of guaiacyl units and related aromatic compounds. The identification of the involved genes unlocks new potential for engineering aromatic compound-producing fungal cell factories.
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