Evidence map›Paper›PMID 34343911›Full record

ArticleColloids and surfaces. B, Biointerfaces2021

Binding kinetics of liposome conjugated E-selectin and P-selectin glycoprotein ligand-1 measured with atomic force microscopy.

Yuyuan Zhou, Wenpeng Cao, Zhiliang Xu, X Frank Zhang, Yaling Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Colloids and surfaces. B, Biointerfaces, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. CD34Frontiers in cell and developmental biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yuyuan ZhouDepartment of Bioengineering, Lehigh University, Bethlehem, PA, 18015, USA.
Wenpeng CaoDepartment of Bioengineering, Lehigh University, Bethlehem, PA, 18015, USA.
Zhiliang XuDepartment of Applied and Computational Mathematics and Statistics, Notre Dame University, Notre Dame, IN, 46556, USA.
X Frank ZhangDepartment of Bioengineering, Lehigh University, Bethlehem, PA, 18015, USA; Department of Mechanical Engineering & Mechanics, Lehigh University, Bethlehem, PA, 18015, USA.
Yaling LiuDepartment of Bioengineering, Lehigh University, Bethlehem, PA, 18015, USA; Department of Mechanical Engineering & Mechanics, Lehigh University, Bethlehem, PA, 18015, USA. Electronic address: yal310@lehigh.edu.
Lehigh University · USUniversity of Notre Dame · US

Funding

Mechanical regulation of von Willebrand factorR01HL153986 · NHLBI · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ZHANG, XIAOHUI · 2021 to 2024
$2.2M
Supplement: Hemolysis Prediction Software DevelopmentR01HL131750 · NHLBI · LEHIGH UNIVERSITY · PI LIU, YALING · 2017 to 2021
$2.2M
Biomechanics of TIM Protein-Mediated Ebola Virus-Host Cell AdhesionR15AI133634 · NIAID · LEHIGH UNIVERSITY · PI JAGOTA, ANAND, ZHANG, XIAOHUI · 2018 to 2018
$435k
Single-cell analysis of endothelial mechanotransduction mediated by endothelial surface glycocalyxR21HL152348 · NHLBI · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ZHANG, XIAOHUI · 2020 to 2021
$408k
NHLBI NIH HHS R01 HL131750NHLBI NIH HHS R01 HL153986NHLBI NIH HHS R21 HL152348NIAID NIH HHS R15 AI133634
6 · The paper itself

Abstract

Various ligand-functionalized liposomes have been developed for targeted therapies. Typically, the binding properties of the ligands and targeted proteins are measured with surface plasmon resonance (SPR), where the proteins are immobilized on a rigid surface. However, the difference of protein-ligand binding kinetics between liposome-conjugated protein and rigid surface-conjugated protein is not fully understood. In this work, the binding kinetics of P-selectin glycoprotein ligand-1 (PSGL-1) and E-selectin conjugated on liposome and on rigid surfaces are investigated with Atomic Force Microscopy (AFM). The results suggest that protein orientation and diffusion on liposomal membrane can alter the binding kinetics of the protein-ligand interaction. Specifically, the association and dissociation rate constant of AFM probe-conjugated E-selectin and glass-conjugated PSGL-1 are measured as 9.32 × 10

Indexed as

E-SelectinLiposomesKineticsLigandsMembrane GlycoproteinsMicroscopy, Atomic ForceProtein BindingE-SelectinLigandsLiposomesMembrane GlycoproteinsP-selectin ligand proteinAtomic force microscopyBinding affinityBinding kineticsE-selectinLiposome

Identifiers

PMID34343911
PMCPMC8487949
OpenAlexW3186929172

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.