Evidence map›Paper›PMID 34343292›Full record

Trial reportThe Journal of clinical endocrinology and metabolism2021

Pilot Trial of Vitamin D3 and Calcifediol in Healthy Vitamin D Deficient Adults: Does It Change the Fecal Microbiome?

Albert Shieh, S Melanie Lee, Venu Lagishetty, Carter Gottleib, Jonathan P Jacobs, John S Adams

Open access · greenAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of clinical endocrinology and metabolism, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Albert ShiehDepartment of Medicine, David Geffen School of Medicine University of California, Los Angeles, CA, USA.ORCID 0000-0002-0695-7976
S Melanie LeeDepartment of Psychiatry, David Geffen School of Medicine University of California, Los Angeles, CA, USA.
Venu LagishettyVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Carter GottleibDepartments of Orthopaedic Surgery and Medicine, David Geffen School of Medicine and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
Jonathan P JacobsVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
John S AdamsDepartments of Orthopaedic Surgery and Medicine, David Geffen School of Medicine and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
University of California, Los Angeles · US

Funding

DBP and the bioavailability and function of vitamin DR01AR063910 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ADAMS, JOHN S, HEWISON, MARTIN · 2013 to 2017
$1.8M
Impact of ethnicity in the gut microbiome in response to oral vitamin D replacementR21AR073018 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ADAMS, JOHN S · 2017 to 2017
$302k
CSRD VA IK2 CX001717NIAMS NIH HHS 5R01AR063910NIAMS NIH HHS R01 AR063910NIAMS NIH HHS R21 AR073018NIH HHS 1R21AR073018VA 5IK2CX001717
6 · The paper itself

Abstract

contextExperimental studies suggest that vitamin D receptor signaling may benefit the gut microbiome. In humans, whether vitamin D supplementation directly alters the gut microbiome is not well studied.

objectiveTo determine whether correcting vitamin D deficiency with cholecalciferol (vitamin D3, D3) or calcifediol (25-hydroxyvitamin D3, 25(OH)D3) changes gut microbiome composition.

methods18 adults with vitamin D deficiency (25-hydroxyvitamin D [25(OH)D] <20 ng/mL) received 60 µg/day of D3 or 20 µg/day of 25(OH)D3 for 8 weeks. Changes in serum 25(OH)D, 1,25-diydroxyvitamin D (1,25(OH)2D), and 24,25-dihydroxyvitamin D (24,25(OH)2D) were assessed. We characterized composition of the fecal microbiota using 16S rRNA gene sequencing, and examined changes in α-diversity (Chao 1, Faith's Phylogenetic Diversity, Shannon Index), β-diversity (DEICODE), and genus-level abundances (DESeq2).

resultsVitamin D3 and 25(OH)D3 groups were similar. After 8 weeks of vitamin D3, mean 25(OH)D and 24,25(OH)2D increased significantly, but 1,25(OH)2D did not (25(OH)D: 17.8-30.1 ng/mL, P = .002; 24,25(OH)2D: 1.1 to 2.7 ng/mL, P =0.003; 1,25(OH)2D: 49.5-53.0 pg/mL, P = .9). After 8 weeks of 25(OH)D3, mean 25(OH)D, 24,25(OH)2D, and 1,25(OH)2D increased significantly (25(OH)D: 16.7-50.6 ng/mL, P < .0001; 24,25(OH)2D: 1.3-6.2 ng/mL, P = .0001; 1,25(OH)2D: 56.5-74.2 pg/mL, P = .05). Fecal microbial α-diversity and β-diversity did not change with D3 or 25D3 supplementation. Mean relative abundance of Firmicutes increased and mean relative abundance of Bacterioidetes decreased from baseline to 4 weeks, but returned to baseline by study completion. DESeq2 analysis did not confirm any statistically significant taxonomic changes.

conclusionIn a small sample of healthy adults with vitamin D deficiency, restoration of vitamin D sufficiency with vitamin D3 or 25(OH)D3 did not lead to lasting changes in the fecal microbiota.

Indexed as

Gastrointestinal MicrobiomeAdolescentAdultBiomarkersCalcifediolCholecalciferolDietary SupplementsFecesFemaleFollow-Up StudiesHumansMalePilot ProjectsPrognosisVitamin D DeficiencyVitaminsBiomarkersCalcifediolCholecalciferolVitaminscalcifediolCholecalciferolgut microbiome

Identifiers

PMID34343292
PMCPMC8864755
OpenAlexW3190097493

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.