ArticleDevelopment (Cambridge, England)2021
Single-cell morphometrics reveals ancestral principles of notochord development.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Positional information and information flows in dynamic tissues.bioRxiv : the preprint server for biology · 2026Article
- A computational pipeline for spatial mechano-transcriptomics.Nature methods · 2025Article
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- Mesodermal fibronectin controls cell shape, polarity, and mechanotransduction in the second heart field during cardiac outflow tract development.Developmental cell · 2025Article
- Building a molecular reference map of the human embryo.Nature methods · 2025Article
- Single-cell Transcriptomic Studies Unveil Potential Nodes of the Notochord Gene Regulatory Network.Integrative and comparative biology · 2024Review
- Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species.Molecular biology and evolution · 2024Article
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- The ion channel Anoctamin 10/TMEM16K coordinates organ morphogenesis across scales in the urochordate notochord.PLoS biology · 2024Article
- Genetic and developmental divergence in the neural crest programme between cichlid fish species.bioRxiv : the preprint server for biology · 2024Article
- An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm.Nature communications · 2024Article
- Spherical harmonics analysis reveals cell shape-fate relationships in zebrafish lateral line neuromasts.Development (Cambridge, England) · 2024Article
- Cell shape characterization, alignment, and comparison using FlowShape.Bioinformatics (Oxford, England) · 2023Article
- Cell state transitions: catch them if you can.Development (Cambridge, England) · 2023Article
- 3D cellular morphometrics of ovule primordium development inFrontiers in plant science · 2023Article
- Machine learning enhanced cell tracking.Frontiers in bioinformatics · 2023Article
- Article
- Functions of the FGF signalling pathway in cephalochordates provide insight into the evolution of the prechordal plate.Development (Cambridge, England) · 2022Article
- Restricted Proliferation During Neurogenesis Contributes to Regionalisation of the Amphioxus Nervous System.Frontiers in neuroscience · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Embryonic tissues are shaped by the dynamic behaviours of their constituent cells. To understand such cell behaviours and how they evolved, new approaches are needed to map out morphogenesis across different organisms. Here, we apply a quantitative approach to learn how the notochord forms during the development of amphioxus: a basally branching chordate. Using a single-cell morphometrics pipeline, we quantify the geometries of thousands of amphioxus notochord cells, and project them into a common mathematical space, termed morphospace. In morphospace, notochord cells disperse into branching trajectories of cell shape change, revealing a dynamic interplay between cell shape change and growth that collectively contributes to tissue elongation. By spatially mapping these trajectories, we identify conspicuous regional variation, both in developmental timing and trajectory topology. Finally, we show experimentally that, unlike ascidians but like vertebrates, posterior cell division is required in amphioxus to generate full notochord length, thereby suggesting this might be an ancestral chordate trait that is secondarily lost in ascidians. Altogether, our novel approach reveals that an unexpectedly complex scheme of notochord morphogenesis might have been present in the first chordates. This article has an associated 'The people behind the papers' interview.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.