Evidence map›Paper›PMID 34343188›Full record

ArticlePloS one2021

Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype.

Amanda Gleyce Lima de Oliveira, Adriana Pittella Sudré, Teresa Cristina Bergamo do Bomfim, Helena Lúcia Carneiro Santos

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

  1. Molecular detection and zoonotic potential ofFood and waterborne parasitology · 2026
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  3. Molecular genetic characterization ofFrontiers in parasitology · 2025
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  5. Evidence of the Zoonotic Transmission ofPathogens (Basel, Switzerland) · 2023
    Article
  6. Article
  7. Current research in parasitology & vector-borne diseases · 2023
    Review
  8. The detection and phylogenetic characterization ofFrontiers in cellular and infection microbiology · 2023
    Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Amanda Gleyce Lima de OliveiraUniversidade Federal Rural do Rio de Janeiro-Departamento de Parasitologia-Instituto de Veterinária-Instituto de Veterinária, Seropédica, Rio de Janeiro, Brazil.
Adriana Pittella SudréUniversidade Federal Fluminense-Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Niterói, Rio de Janeiro, Brazil.
Teresa Cristina Bergamo do BomfimUniversidade Federal Rural do Rio de Janeiro-Departamento de Parasitologia-Instituto de Veterinária-Instituto de Veterinária, Seropédica, Rio de Janeiro, Brazil.ORCID 0000-0002-6741-0202
Helena Lúcia Carneiro SantosInstituto Oswaldo Cruz-Laboratório de Estudos Integrados em Protozoologia, Rio de Janeiro, Brazil.
Universidade Federal Rural do Rio de Janeiro · BRFaculdades Oswaldo Cruz · BRUniversidade Federal Fluminense · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal cryptosporidiosis is a diarrheal disease caused by protists of genus Cryptosporidium that infect a wide variety of hosts, primarily vertebrates. Due to the close contact between humans and their companion animals, especially dogs and cats, there is concern about the potential for zoonotic transmission of this enteric protozoan parasite by infected animals. This study aimed to perform a microscopic and molecular diagnosis of Cryptosporidium spp. in fecal samples from domiciled dogs and cats. One hundred and nineteen fecal samples were processed using sugar centrifugal flotation followed by molecular detection of Cryptosporidium spp. DNA using nested PCR. Subtyping of isolates positive for C. parvum was performed by sequence analysis of the 60 kDa glycoprotein gene (GP60). Cryptosporidium oocysts were detected in 7.8% (5/64) and 5.4% (3/55) of the fecal samples from dogs and cats, respectively. Cryptosporidium canis (n = 3) and C. parvum (n = 2) were the main species found in dogs, whereas C. felis (n = 3) was prevalent in cats. Subtype IIaA17G2R2 (potentially zoonotic) was identified in samples positive for C. parvum. Despite the low prevalence of Cryptosporidium observed in the domiciled dogs and cats, the presence of potentially zoonotic C. parvum in dogs evidences a public health concern. Further research is needed to better understand the epidemiology, source, and potential impacts of Cryptosporidium infection in cats and dogs.

Indexed as

AnimalsBrazilCatsCryptosporidiumDogsFecesFemaleGenotypeGeographyLikelihood FunctionsMalePhylogenyZoonoses

Identifiers

PMID34343188
PMCPMC8330930
OpenAlexW3191666853

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.