Evidence map›Paper›PMID 34342815›Full record

ArticleMolecular biology reports2021

Insertion of prpoD_rpoS fragment enhances expression of recombinant protein by dps auto-inducible promoter in Escherichia coli.

Dina Mulyanti, Sundani Nurono Soewandhi, Catur Riani

Abstract read
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In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Dina MulyantiSchool of Pharmacy, Bandung Institute of Technology, Ganesha 10, Bandung, 40132, Indonesia.ORCID http://orcid.org/0000-0002-9555-3200
Sundani Nurono SoewandhiSchool of Pharmacy, Bandung Institute of Technology, Ganesha 10, Bandung, 40132, Indonesia.
Catur RianiSchool of Pharmacy, Bandung Institute of Technology, Ganesha 10, Bandung, 40132, Indonesia. catur@fa.itb.ac.id.ORCID http://orcid.org/0000-0001-7082-0264
Bandung Institute of Technology · ID

Funding

Indonesia Toray Science Foundation Science & Technology Research Grant (2017)Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia (ID) Hibah Kompetensi (2018-2019)
6 · The paper itself

Abstract

backgroundNowadays, recombinant therapeutic proteins have been widely produced and consumed. For the safety and effectiveness of the protein production, an auto-inducible expression vector is required to replace inducer interference, which is uneconomic and could be harmful. In this research, an auto-inducible expression plasmid, pCAD METHOD AND

resultsA synthetic rpoS coding region under rpoD promoter control (prpoD_rpoS) was inserted to pCAD

conclusionsThe prpoD_rpoS insertion shifts and increases the rMnSODSeq production from stationary to exponential phase. The pCAD

Indexed as

Bacterial Outer Membrane ProteinsBacterial ProteinsDNA-Binding ProteinsEscherichia coliEscherichia coli ProteinsGene ExpressionGene Expression Regulation, BacterialGenetic VectorsPlasmidsPromoter Regions, GeneticProtein EngineeringRecombinant ProteinsSigma FactorSuperoxide DismutaseBacterial Outer Membrane ProteinsBacterial ProteinsDNA-Binding Proteinsdps protein, E coliEscherichia coli ProteinsRecombinant ProteinsSigma FactorSuperoxide DismutaseAuto-inducibledps promoterRpoSSuperoxide dismutase

Identifiers

PMID34342815
OpenAlexW3128930176

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.