Evidence map›Paper›PMID 34342711›Full record

ArticleApplied microbiology and biotechnology2021

Efficient synthesis of bepotastine and cloperastine intermediates using engineered alcohol dehydrogenase with a hydrophobic pocket.

Kai Wu, Jinrong Yan, Xiaojing Wang, Xinai Yin, Guixiang Shi, Lei Yang, Fangling Li, Junhai Huang, Lei Shao

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Kai WuSchool of Pharmacy, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Jinrong YanCollege of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai, 201620, China.
Xiaojing WangMicrobial Pharmacology Laboratory, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Xinai YinMicrobial Pharmacology Laboratory, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Guixiang ShiSchool of Pharmacy, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Lei YangSchool of Pharmacy, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Fangling LiSchool of Pharmacy, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China.
Junhai HuangState Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, 285 Gebaini Rd, Shanghai, 200040, China.
Lei ShaoMicrobial Pharmacology Laboratory, Shanghai University of Medicine & Health sciences, 279 Zhouzhu Highway, Pudong New Area, Shanghai, 201318, China. shaolei00@gmail.com.ORCID http://orcid.org/0000-0003-4130-2817
Shanghai University of Medicine and Health Sciences · CNShanghai Institute of Pharmaceutical Industry · CNShanghai University of Engineering Science · CN

Funding

Natural Science Foundation of China 81773616Natural Science Foundation of Shanghai 20ZR1424600Shanghai Sailing Program 20YF1417900
6 · The paper itself

Abstract

(S)-4-Chlorophenylpyridylmethanol and (R)-4-chlorobenzhydrol are key pharmaceutical intermediates for the synthesis of bepotastine and cloperastine, respectively. However, the biocatalytic approach to prepare these bulky diaryl ketones remains challenging because of the low activity of naturally occurring alcohol dehydrogenases (ADH). In the present study, ADH seq5, which has an adequate binding pocket volume and accepts bulky diaryl ketones, was further engineered with a binding pocket of increased hydrophobicity. Based on molecular simulation and binding free energy analyses, a small mutation library was constructed, and mutant seq5-D150I with a threefold increase in k

Indexed as

Alcohol DehydrogenasePiperidinesBiocatalysisHydrophobic and Hydrophilic InteractionsKineticsPyridinesStereoisomerismAlcohol DehydrogenasebepotastinecloperastinePiperidinesPyridinesAlcohol dehydrogenaseAsymmetric bioreductionBiocatalysisEngineering

Identifiers

PMID34342711
OpenAlexW3188254216

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.