Evidence map›Paper›PMID 34341794›Full record

ArticlebioRxiv : the preprint server for biology2021

Differential Interactions Between Human ACE2 and Spike RBD of SARS-CoV-2 Variants of Concern.

Seonghan Kim, Yi Liu, Zewei Lei, Jeffrey Dicker, Yiwei Cao, X Frank Zhang, Wonpil Im

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 39 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Seonghan KimDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Yi LiuDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Zewei LeiDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Jeffrey DickerDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Yiwei CaoDepartments of Biological Sciences, Chemistry, and Computer Science and Engineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
X Frank ZhangDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Wonpil ImDepartment of Bioengineering, Lehigh University, 111 Research Dr, Bethlehem, PA 18015, USA.
Lehigh University · US

Funding

CHARMM-GUI Development for Biomolecular Modeling and Simulation CommunityR01GM138472 · NIGMS · LEHIGH UNIVERSITY · PI IM, WONPIL · 2020 to 2023
$1.5M
Biomechanics of TIM Protein-Mediated Ebola Virus-Host Cell AdhesionR15AI133634 · NIAID · LEHIGH UNIVERSITY · PI JAGOTA, ANAND, ZHANG, XIAOHUI · 2018 to 2018
$435k
Biophysical characterization of SARS-CoV-2 spike protein - receptor interactionsR21AI163708 · NIAID · LEHIGH UNIVERSITY · PI IM, WONPIL, ZHANG, XIAOHUI · 2021 to 2022
$426k
NIAID NIH HHS R15 AI133634NIAID NIH HHS R21 AI163708NIGMS NIH HHS R01 GM138472
6 · The paper itself

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current coronavirus disease 2019 (COVID-19) pandemic. It is known that the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 interacts with the human angiotensin-converting enzyme 2 (ACE2) receptor, initiating the entry of SARS-CoV-2. Since its emergence, a number of SARS-CoV-2 variants have been reported, and the variants that show high infectivity are classified as the variants of concern according to the US CDC. In this study, we performed both all-atom steered molecular dynamics (SMD) simulations and microscale thermophoresis (MST) experiments to characterize the binding interactions between ACE2 and RBD of all current variants of concern (Alpha, Beta, Gamma, and Delta) and two variants of interest (Epsilon and Kappa). We report that the RBD of the Alpha (N501Y) variant requires the highest amount of force initially to be detached from ACE2 due to the N501Y mutation in addition to the role of N90-glycan, followed by Beta/Gamma (K417N/T, E484K, and N501Y) or Delta (L452R and T478K) variant. Among all variants investigated in this work, the RBD of the Epsilon (L452R) variant is relatively easily detached from ACE2. Our results combined SMD simulations and MST experiments indicate what makes each variant more contagious in terms of RBD and ACE2 interactions. This study could help develop new drugs to inhibit SARS-CoV-2 entry effectively. ABSTRACT FIGURE:

Identifiers

PMID34341794
PMCPMC8328061
OpenAlexW3185068335

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.