ArticleTranslational biophotonics2020
Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.
Article in Translational biophotonics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.Journal of biophotonics · 2025Pooled it
- Third harmonic generation microscopy reveals structure and mucus dynamics in human airway epithelium models.iScience · 2026Article
- Short-pulsed AI-augmented third harmonic generation imaging visualizes negative-contrast cell nuclei in fresh mouse liver tissue without optical-induced damage.Biomedical optics express · 2026Article
- Deep learning-based 3D leukocyte differentiation using label-free higher harmonic generation microscopy.Journal of translational medicine · 2026Article
- 4D Time-lapse imaging of cell dynamics in human alveolar tissue by label-free higher harmonic generation microscopy.Journal of translational medicine · 2026Article
- Intraoperative biopsy imaging of lung cancer risk.Communications medicine · 2026Article
- Multi-Observer Study on Diagnostic Accuracy of Pediatric Renal Tumors Imaged with Higher-Harmonic-Generation Microscopy.Cancers · 2025Article
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- Current Landscape of Advanced Imaging Tools for Pathology Diagnostics.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024Review
- Modeling the extracellular matrix in cell migration and morphogenesis: a guide for the curious biologist.Frontiers in cell and developmental biology · 2024Review
- Rapid On-Site Pathology Visualization of COVID-19 Characteristics Using Higher Harmonic Generation Microscopy.American journal of respiratory and critical care medicine · 2023Article
- Leukocyte differentiation in bronchoalveolar lavage fluids using higher harmonic generation microscopy and deep learning.PloS one · 2023Article
- Fast intraoperative histology-based diagnosis of gliomas with third harmonic generation microscopy and deep learning.Scientific reports · 2022Article
- Non-Invasive Skin Imaging Assessment of Human Stress During Head-Down Bed Rest Using a Portable Handheld Two-Photon Microscope.Frontiers in physiology · 2022Article
- Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.Translational biophotonics · 2020Article
- Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During lung cancer operations a rapid and reliable assessment of tumor tissue can reduce operation time and potentially improve patient outcomes. We show that third harmonic generation (THG), second harmonic generation (SHG) and two-photon excited autofluorescence (2PEF) microscopy reveals relevant, histopathological information within seconds in fresh unprocessed human lung samples. We used a compact, portable microscope and recorded images within 1 to 3 seconds using a power of 5 mW. The generated THG/SHG/2PEF images of tumorous and nontumorous tissues are compared with the corresponding standard histology images, to identify alveolar structures and histopathological hallmarks. Cellular structures (tumor cells, macrophages and lymphocytes) (THG), collagen (SHG) and elastin (2PEF) are differentiated and allowed for rapid identification of carcinoid with solid growth pattern, minimally enlarged monomorphic cell nuclei with salt-and-pepper chromatin pattern, and adenocarcinoma with lipidic and micropapillary growth patterns. THG/SHG/2PEF imaging is thus a promising tool for clinical intraoperative assessment of lung tumor tissue.
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Registered trials
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