ArticleNature chemical biology2021
Designer membraneless organelles sequester native factors for control of cell behavior.
Article in Nature chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 146 citations in OpenAlex.
- FAIDR-Desi: interactive software for feature analysis and design of intrinsically disordered regions.Bioinformatics (Oxford, England) · 2026Article
- Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology.Synthetic and systems biotechnology · 2026Review
- Directed evolution of functional intrinsically disordered proteins.Nature chemical biology · 2026Article
- Hydration free energy is an incomplete predictor of globular protein incorporation into condensates.Biophysical journal · 2026Article
- Cellular Morphometric Analysis (CellMorph)-a comprehensive imaging-based tool for quantifying cellular phenotype heterogeneity and dynamics across biological processes.The FEBS journal · 2026Article
- Beyond The Nucleus: Translating Engineered Protein Localization To Chromatin Modifying Enzymes.Current opinion in biomedical engineering · 2026Article
- An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cells.bioRxiv : the preprint server for biology · 2026Article
- Review
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- Expression of nano-engineered RNA organelles in bacteria.Nature communications · 2026Article
- Cross-scale design of abiotic-biotic interfaces for semi-artificial photosynthesis.Chemical science · 2026Review
- Delivery of peptide coacervates to form stable interaction hubs in cells.Nature communications · 2026Article
- Repurposing nuclear receptors for ligand-responsive liquid condensate formation and gene regulation.Nature communications · 2026Article
- Stimuli-triggered formation ofCell biomaterials · 2026Article
- Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Condensatopathies as a mechanistic framework for disease and integrated theranostic intervention.Theranostics · 2026Review
- Synthetic Biomolecular Condensates: Design Principles and Applications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Construction of artificial organellesChemical science · 2025Review
- Engineered membraneless organelles inSynthetic and systems biotechnology · 2025Article
- Molecular motor-driven reversible liquid-liquid phase separation of supramolecular assemblies.Nature communications · 2025Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Subcellular compartmentalization of macromolecules increases flux and prevents inhibitory interactions to control biochemical reactions. Inspired by this functionality, we sought to build designer compartments that function as hubs to regulate the flow of information through cellular control systems. We report a synthetic membraneless organelle platform to control endogenous cellular activities through sequestration and insulation of native proteins. We engineer and express a disordered protein scaffold to assemble micron-size condensates and recruit endogenous clients via genomic tagging with high-affinity dimerization motifs. By relocalizing up to 90% of targeted enzymes to synthetic condensates, we efficiently control cellular behaviors, including proliferation, division and cytoskeletal organization. Further, we demonstrate multiple strategies for controlled cargo release from condensates to switch cells between functional states. These synthetic organelles offer a powerful and generalizable approach to modularly control cell decision-making in a variety of model systems with broad applications for cellular engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.