ArticleNature protocols2021
High-speed laser-scanning biological microscopy using FACED.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Patch deconvolution for Fourier light-field microscopy.Biophysical journal · 2026Article
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice.PhotoniX · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Single-pixel imaging flow cytometry for biomedical research.Inflammation and regeneration · 2025Review
- Generalizable morphological profiling of cells by interpretable unsupervised learning.Nature communications · 2025Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
- Suite3D: Volumetric cell detection for two-photon microscopy.bioRxiv : the preprint server for biology · 2025Article
- Imaging flow cytometry with a real-time throughput beyond 1,000,000 events per second.Light, science & applications · 2025Article
- Improving the scan throughput of polygon scanners.Biomedical optics express · 2024Article
- High-throughput fluorescence lifetime imaging flow cytometry.Nature communications · 2024Article
- Information-Distilled Generative Label-Free Morphological Profiling Encodes Cellular Heterogeneity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- High-speed optical imaging with sCMOS pixel reassignment.Nature communications · 2024Article
- Light-field flow cytometry for high-resolution, volumetric and multiparametric 3D single-cell analysis.Nature communications · 2024Article
- Artificial intelligence-enabled quantitative phase imaging methods for life sciences.Nature methods · 2023Review
- Machine learning implementation strategy in imaging and impedance flow cytometry.Biomicrofluidics · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Laser scanning is used in advanced biological microscopy to deliver superior imaging contrast, resolution and sensitivity. However, it is challenging to scale up the scanning speed required for interrogating a large and heterogeneous population of biological specimens or capturing highly dynamic biological processes at high spatiotemporal resolution. Bypassing the speed limitation of traditional mechanical methods, free-space angular-chirp-enhanced delay (FACED) is an all-optical, passive and reconfigurable laser-scanning approach that has been successfully applied in different microscopy modalities at an ultrafast line-scan rate of 1-80 MHz. Optimal FACED imaging performance requires optimized experimental design and implementation to enable specific high-speed applications. In this protocol, we aim to disseminate information allowing FACED to be applied to a broader range of imaging modalities. We provide (i) a comprehensive guide and design specifications for the FACED hardware; (ii) step-by-step optical implementations of the FACED module including the key custom components; and (iii) the overall image acquisition and reconstruction pipeline. We illustrate two practical imaging configurations: multimodal FACED imaging flow cytometry (bright-field, fluorescence and second-harmonic generation) and kHz 2D two-photon fluorescence microscopy. Users with basic experience in optical microscope operation and software engineering should be able to complete the setup of the FACED imaging hardware and software in ~2-3 months.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.