ArticleThe ISME journal2022
A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Planktonic anammox bacteria toward a better understanding of ecophysiological traits and harnessing the untapped potential as a bioresource.Bioprocess and biosystems engineering · 2026Review
- Wastewater metaproteomics: tracking microbial and human protein biomarkers.ISME communications · 2026Article
- Identification of a distinct sialic acid (KDN) and a KDN-specific aldolase in Pacific oyster.Glycoconjugate journal · 2025Article
- Exploring proteinmBio · 2025Article
- Microbial extracellular polymeric substances in the environment, technology and medicine.Nature reviews. Microbiology · 2025Review
- Article
- Sweet Secrets: Exploring Novel Glycans and Glycoconjugates in the Extracellular Polymeric Substances of "ACS ES&T water · 2024Article
- Full-length single-molecule protein fingerprinting.Nature nanotechnology · 2024Article
- Predicted metabolic roles and stress responses provide insights into candidate phyla Hydrogenedentota and Sumerlaeota as members of the rare biosphere in biofilms from various environments.Environmental microbiology reports · 2024Article
- Osmoregulation in freshwater anaerobic methane-oxidizing archaea under salt stress.The ISME journal · 2024Article
- NovoLign: metaproteomics by sequence alignment.ISME communications · 2024Article
- Quantitative proteomics reveals oxygen-induced adaptations inFrontiers in microbiology · 2024Article
- Characterization of protein glycosylation in an Asgard archaeon.BBA advances · 2024Article
- Physiological and stoichiometric characterization of ethanol-based chain elongation in the absence of short-chain carboxylic acids.Scientific reports · 2023Article
- Recent trends in glycoproteomics by characterization of intact glycopeptides.Analytical and bioanalytical chemistry · 2023Review
- Surface-layer protein is a public-good matrix exopolymer for microbial community organisation in environmental anammox biofilms.The ISME journal · 2023Article
- Membrane proteome of the thermoalkaliphileFrontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains of life, the understanding of their biological significance and the evolutionary forces shaping oligosaccharide structures remains highly limited. Here, we report on a newly established mass binning glycoproteomics approach that establishes the chemical identity of the carbohydrate components and performs untargeted exploration of prokaryotic oligosaccharides from large-scale proteomics data directly. We demonstrate our approach by exploring an enrichment culture of the globally relevant anaerobic ammonium-oxidizing bacterium Ca. Kuenenia stuttgartiensis. By doing so we resolve a remarkable array of oligosaccharides, which are produced by two seemingly unrelated biosynthetic routes, and which modify the same surface-layer protein simultaneously. More intriguingly, the investigated strain also accomplished modulation of highly specialized sugars, supposedly in response to its energy metabolism-the anaerobic oxidation of ammonium-which depends on the acquisition of substrates of opposite charges. Ultimately, we provide a systematic approach for the compositional exploration of prokaryotic protein glycosylation, and reveal a remarkable example for the evolution of complex oligosaccharides in bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.