ArticlemBio2021
The dCache Chemoreceptor TlpA of Helicobacter pylori Binds Multiple Attractant and Antagonistic Ligands via Distinct Sites.
Article in mBio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- 4-hydroxy-5-methyl-3(2H)-furanone mediates widespread interkingdom communications between fungi and prokaryotes.Genome biology · 2026Article
- Effects of Helicobacter pylori infection on gastric mucosal microbiota.World journal of pediatrics : WJP · 2026Review
- Chemotaxis to plant defense compounds in phytopathogens.PLoS pathogens · 2026Article
- Pathogenicity and virulence ofVirulence · 2025Review
- Structural and functional diversity of sensor domains in bacterial transmembrane receptors.Trends in microbiology · 2025Review
- Low pH amplifies chemotaxis toward urea in Helicobacter pylori.Biophysical journal · 2025Article
- Bacterial sensor evolved by decreasing complexity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Urea-Loaded PLGA Microspheres as Chemotaxis Stimulants for Helicobacter pylori.Biotechnology and bioengineering · 2025Article
- Bacterial amino acid chemotaxis: a widespread strategy with multiple physiological and ecological roles.Journal of bacteriology · 2024Review
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- Identification of a dCache-type chemoreceptor inFrontiers in microbiology · 2024Article
- The importance of cache domains in αChannels (Austin, Tex.) · 2023Article
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- Helicobacter pylori Chronic-Stage Inflammation Undergoes Fluctuations That Are Altered inInfection and immunity · 2023Article
- Diverse Sensory Repertoire of Paralogous Chemoreceptors Tlp2, Tlp3, and Tlp4 inMicrobiology spectrum · 2022Article
- Multiple detection of both attractants and repellents by the dCache-chemoreceptor SO_1056 of Shewanella oneidensis.The FEBS journal · 2022Article
- Signal binding at both modules of its dCache domain enables the McpA chemoreceptor ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Amino acid sensor conserved from bacteria to humans.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The Helicobacter pylori chemoreceptor TlpA plays a role in dampening host inflammation during chronic stomach colonization. TlpA has a periplasmic dCache_1 domain, a structure that is capable of sensing many ligands; however, the only characterized TlpA signals are arginine, bicarbonate, and acid. To increase our understanding of TlpA's sensing profile, we screened for diverse TlpA ligands using ligand binding arrays. TlpA bound seven ligands with affinities in the low- to middle-micromolar ranges. Three of these ligands, arginine, fumarate, and cysteine, were TlpA-dependent chemoattractants, while the others elicited no response. Molecular docking experiments, site-directed point mutants, and competition surface plasmon resonance binding assays suggested that TlpA binds ligands via both the membrane-distal and -proximal dCache_1 binding pockets. Surprisingly, one of the nonactive ligands, glucosamine, acted as a chemotaxis antagonist, preventing the chemotaxis response to chemoattractant ligands, and acted to block the binding of ligands irrespective of whether they bound the membrane-distal or -proximal dCache_1 subdomains. In total, these results suggest that TlpA senses multiple attractant ligands as well as antagonist ones, an emerging theme in chemotaxis systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.