Evidence map›Paper›PMID 34339765›Full record

ArticleJournal of virological methods2021

Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances.

Matías Salvo, Ana Moller, Elena Alvareda, Pablo Gamazo, Rodney Colina, Matías Victoria

Abstract read
In one paragraph

Article in Journal of virological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Optimized Aluminum Hydroxide Adsorption-Precipitation for Improved Viral Detection in Wastewater.International journal of environmental research and public health · 2025
    Article
  4. Article
  5. Review
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  7. Article
  8. Article
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  11. Assessment of virus concentration methods for detecting SARS-CoV-2 IN wastewater.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023
    Article
  12. Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Matías SalvoLaboratory of Molecular Virology, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay; Water Department, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay.
Ana MollerLaboratory of Molecular Virology, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay.
Elena AlvaredaWater Department, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay.
Pablo GamazoWater Department, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay.
Rodney ColinaLaboratory of Molecular Virology, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay.
Matías VictoriaLaboratory of Molecular Virology, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350, Salto CP: 50.000, Uruguay. Electronic address: matvicmon@yahoo.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) many strategies have been performed in order to control viral spread in the population and known the real-time situation about the number of infected persons. In this sense, Wastewater Based Epidemiology (WBE) has been applied as an excellent tool to evaluate the virus circulation in a population. In order to obtain reliable results, three low-cost viral concentration methods were evaluated in this study, polyethylene glycol (PEG) precipitation, skimmed milk flocculation (SM) and Aluminum polychloride flocculation, for Pseudomonas aeruginosa bacteriophage PP7 as a surrogate for non-enveloped viruses and Bovine Coronavirus (BCoV) as a surrogate for enveloped virus, with emphasis for SARS- CoV-2. Our results suggest that PEG precipitation for viral concentration, for both enveloped and non-enveloped virus from wastewater is an appropriate approach since it was more sensitive compared to SM flocculation and Aluminum polychloride flocculation. This methodology can be used for WBE studies in order to follow the epidemiology of the SARS-CoV-2 pandemic, mainly in developing countries where the economic resources are frequently limited.

Indexed as

COVID-19VirusesAnimalsCattleHumansPandemicsSARS-CoV-2WastewaterWastewaterAluminum polychloride flocculationBCoVPolyethylene glycol precipitationPP7Skimmed milk flocculation

Identifiers

PMID34339765
PMCPMC8324412

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.