ArticleAngewandte Chemie (International ed. in English)2021
Strategies to Avoid Artifacts in Mass Spectrometry-Based Epitranscriptome Analyses.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Article
- Harmonized nucleoside mass spectrometry enables reproducible cross-platform RNA modification quantification.bioRxiv : the preprint server for biology · 2026Article
- Long-term stability of RNA nucleoside standards for accurate LC-MS quantification.Nucleic acids research · 2026Article
- Sulfur and Selenium Modifications at Phosphorus Atom in Nucleoside Monophosphates, Activity and Potential Applications.Current medicinal chemistry · 2026Review
- Pharmaceutical perspectives on oligonucleotide therapeutics and delivery systems.Pharmacological reviews · 2025Review
- Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome.Microbiome · 2025Article
- Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome.bioRxiv : the preprint server for biology · 2024Article
- Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery.International journal of molecular sciences · 2024Review
- Reversible oxidative dimerization of 4-thiouridines in tRNA isolates.RSC chemical biology · 2024Article
- RNA modifications in physiology and disease: towards clinical applications.Nature reviews. Genetics · 2024Review
- Spacer Fidelity Assessments of Guide RNA by Top-Down Mass Spectrometry.ACS central science · 2023Article
- Chemistry, structure and function of approved oligonucleotide therapeutics.Nucleic acids research · 2023Review
- Opportunities and Challenges to Profile mRNA Modifications in Escherichia coli.Chembiochem : a European journal of chemical biology · 2022Article
- Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.Nature communications · 2022Article
- Review
- Article
- Review
- Strategies to Avoid Artifacts in Mass Spectrometry-Based Epitranscriptome Analyses.Angewandte Chemie (International ed. in English) · 2021Article
- Mass Spectrometry Analysis of Nucleic Acid Modifications: From Beginning to Future.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
In this report, we perform structure validation of recently reported RNA phosphorothioate (PT) modifications, a new set of epitranscriptome marks found in bacteria and eukaryotes including humans. By comparing synthetic PT-containing diribonucleotides with native species in RNA hydrolysates by high-resolution mass spectrometry (MS), metabolic stable isotope labeling, and PT-specific iodine-desulfurization, we disprove the existence of PTs in RNA from E. coli, S. cerevisiae, human cell lines, and mouse brain. Furthermore, we discuss how an MS artifact led to the initial misidentification of 2'-O-methylated diribonucleotides as RNA phosphorothioates. To aid structure validation of new nucleic acid modifications, we present a detailed guideline for MS analysis of RNA hydrolysates, emphasizing how the chosen RNA hydrolysis protocol can be a decisive factor in discovering and quantifying RNA modifications in biological samples.
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Registered trials
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