ArticleThe Plant cell2021
An ABHD17-like hydrolase screening system to identify de-S-acylation enzymes of protein substrates in plant cells.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 22 citations in OpenAlex.
- Reversible S-palmitoylation of C4 protein encoded by TYLCCxV orchestrates geminiviral pathogenesis.Stress biology · 2026Article
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- Phylogenomic Insights into High Conservation and Lineage-Specific Expansion of the ABAPT Gene Family in Plants.International journal of molecular sciences · 2026Article
- An optogenetic transcriptional regulator controlled by reversible S-acylation in plants.Plant communications · 2026Article
- Shared Plant-human Biology: Herbicide Effects and New Biomarkers Perspectives.Current environmental health reports · 2026Review
- De novo transcriptome assembly and gene expression analysis of Cnidium officinale under high-temperature conditions.BMC genomics · 2025Article
- Global analysis of membrane protein S-acylation in the model plantbioRxiv : the preprint server for biology · 2025Article
- Post-Translational Modifications to Cysteine Residues in Plant Proteins and Their Impact on the Regulation of Metabolism and Signal Transduction.International journal of molecular sciences · 2024Review
- Improvement of plant resistance to geminiviruses via protein de-S-acylation.Stress biology · 2024Article
- The zinc finger protein DHHC09 S-acylates the kinase STRK1 to regulate H2O2 homeostasis and promote salt tolerance in rice.The Plant cell · 2024Article
- Salicylic acid attenuates brassinosteroid signaling via protein de-S-acylation.The EMBO journal · 2023Article
- Free Radicals Mediated Redox Signaling in Plant Stress Tolerance.Life (Basel, Switzerland) · 2023Review
- Identification of a candidate dwarfing gene in Pallas, the first commercial barley cultivar generated through mutational breeding.Frontiers in genetics · 2023Article
- Palmitoylation of γb protein directs a dynamic switch between Barley stripe mosaic virus replication and movement.The EMBO journal · 2022Article
- Protein S-acyltransferases and acyl protein thioesterases, regulation executors of protein S-acylation in plants.Frontiers in plant science · 2022Review
- Cutting out the fat: A new screen for de-S-acylases in plants.The Plant cell · 2021Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein S-acylation is an important post-translational modification in eukaryotes, regulating the subcellular localization, trafficking, stability, and activity of substrate proteins. The dynamic regulation of this reversible modification is mediated inversely by protein S-acyltransferases and de-S-acylation enzymes, but the de-S-acylation mechanism remains unclear in plant cells. Here, we characterized a group of putative protein de-S-acylation enzymes in Arabidopsis thaliana, including 11 members of Alpha/Beta Hydrolase Domain-containing Protein 17-like acyl protein thioesterases (ABAPTs). A robust system was then established for the screening of de-S-acylation enzymes of protein substrates in plant cells, based on the effects of substrate localization and confirmed via the protein S-acylation levels. Using this system, the ABAPTs, which specifically reduced the S-acylation levels and disrupted the plasma membrane localization of five immunity-related proteins, were identified respectively in Arabidopsis. Further results indicated that the de-S-acylation of RPM1-Interacting Protein 4, which was mediated by ABAPT8, resulted in an increase of cell death in Arabidopsis and Nicotiana benthamiana, supporting the physiological role of the ABAPTs in plants. Collectively, our current work provides a powerful and reliable system to identify the pairs of plant protein substrates and de-S-acylation enzymes for further studies on the dynamic regulation of plant protein S-acylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.