ArticleCell adhesion & migration2021
Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D.
Deborah J Wessels, Claude Pujol, Nikash Pradhan, Daniel F Lusche, Luis Gonzalez, Sydney E Kelly, Elizabeth M Martin, Edward R Voss, Yang-Nim Park, Michael Dailey and 4 more
Open access · goldAbstract read
In one paragraphArticle in Cell adhesion & migration, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 1 citations in OpenAlex.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
14 authors at 2 institutions in 1 country.
Deborah J WesselsDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.ORCID 0000-0003-2232-894X Claude PujolDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Nikash PradhanDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Daniel F LuscheDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.ORCID 0000-0002-8848-4198 Luis GonzalezDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Sydney E KellyDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Elizabeth M MartinDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Edward R VossDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Yang-Nim ParkDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.
Michael DaileyDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.ORCID 0000-0003-2962-4661 Sonia L SuggDepartment of Surgery, The University of Iowa Hospitals and Clinics, Iowa City, IA, USA.ORCID 0000-0001-7530-8526 Sneha PhadkeDepartment of Internal Medicine, The University of Iowa Hospitals and Clinics, Iowa City, IA, USA.ORCID 0000-0002-9829-1066 Amani BashirDepartment of Pathology, The University of Iowa Hospitals and Clinics, Iowa City, IA, USA.ORCID 0000-0002-7239-3662 David R SollDevelopmental Studies Hybridoma Bank and W.M. Keck Dynamic Image Analysis Facility, Department of Biology, The University of Iowa, Iowa City, IA, USA.ORCID 0000-0002-5059-0315 University of Iowa · USUniversity of Iowa Hospitals and Clinics · US
Funding
Request for Funds to Purchase a Replacement Laser Scanning Confocal MicroscopeS10RR025439 · NCRR · UNIVERSITY OF IOWA · PI ZABNER, JOSEPH · 2009 to 2009
$500kNCRR NIH HHS S10 RR025439
6 · The paper itselfAbstract
We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.
Indexed as
Breast NeoplasmsPharmaceutical PreparationsCell MovementFemaleHumansHuman Umbilical Vein Endothelial CellsPseudopodiaPharmaceutical PreparationsCD44computer-assisted reconstructionextravasationFilopodiaintravasationpseudopodRHAMM
Identifiers
PMID34338608
PMCPMC8331046
OpenAlexW3187237384
What OpenQuestion holds
Textmetadata
LicenceCC BY
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