Evidence map›Paper›PMID 34337864›Full record

ArticleThe Kaohsiung journal of medical sciences2021

Exosomal microRNA-15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis.

Dao-Yuan Li, Fei-Fei Lin, Guo-Ping Li, Fan-Chang Zeng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Kaohsiung journal of medical sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
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  10. Exosomes in the Diagnosis and Treatment of Renal Cell Cancer.International journal of molecular sciences · 2023
    Review
  11. Article
  12. Biomarkers in renal cell carcinoma and their targeted therapies: a review.Exploration of targeted anti-tumor therapy · 2023
    Review
  13. Article
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  15. Review
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dao-Yuan LiDepartment of Urology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, China.ORCID https://orcid.org/0000-0003-4094-6784
Fei-Fei LinDepartment of Otorhinolaryngology - Head and Neck Surgery, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, China.ORCID https://orcid.org/0000-0001-7595-646X
Guo-Ping LiDepartment of Urology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, China.ORCID https://orcid.org/0000-0002-9709-0588
Fan-Chang ZengDepartment of Urology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, China.ORCID https://orcid.org/0000-0002-2010-8495
Hainan General Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accumulating studies have indicated that exosomal microRNAs (miRNAs/miRs) can mediate clear cell renal cell carcinoma (ccRCC) at the early stage, but the mechanisms remain to be specified. Here, we investigated the mechanism of exosomal miR-15a in ccRCC. After successful isolation of exosomes from RCC cells, we found that miR-15a was upregulated in ccRCC cells. Moreover, upregulation of miR-15a by pre-miR-15a promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition of ccRCC cells. A luciferase assay revealed that B-cell translocation gene 2 (BTG2) was a target gene of miR-15a and negatively correlated with miR-15a expression. BTG2 was poorly expressed in ccRCC, which reduced the proliferation of ccRCC cells. In addition, overexpression of BTG2 could reverse the promotive effects of miR-15a on ccRCC. Furthermore, BTG2 reduced PI3K/AKT pathway activity. Our results collectively indicated that exosomal miR-15a from RCC cells accelerated cell viability by downregulating BTG2 and promoting the activity of the PI3K/AKT signaling pathway. We demonstrated a novel mechanism by which exosomal miR-15a exerted pro-proliferatory effects on ccRCC, highlighting the potential of exosomal miR-15a as a target for ccRCC prognosis.

Indexed as

Base PairingBase SequenceCarcinoma, Renal CellCell Line, TumorCell MovementCell ProliferationEpithelial-Mesenchymal TransitionExosomesGene Expression Regulation, NeoplasticGenes, ReporterHumansImmediate-Early ProteinsKidney NeoplasmsLuciferasesMicroRNAsNeoplasm InvasivenessBTG2 protein, humanImmediate-Early ProteinsLuciferasesMicroRNAsMIRN15 microRNA, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTumor Suppressor ProteinsB-cell translocation gene 2clear cell renal cell carcinomaexosomesmicroRNA-15aPI3K/AKT pathway

Identifiers

PMID34337864
PMCPMC11896453
OpenAlexW3189005553

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.