Base editing-coupled survival screening enabled high-sensitive analysis of PAM compatibility and finding of the new possible off-target.

Tianyuan Su, Qi Guo, Yi Zheng, Yizhao Chang, Fei Gu, Xuemei Lu, Qingsheng Qi

Abstract read

In one paragraph

Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

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1 citing paper in PubMed.

Biodegradation of polyurethane by the microbial consortia enriched from landfill.Applied microbiology and biotechnology · 2023
Article

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5 · Who and what money

Authors and funding

7 authors.

Tianyuan SuState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Qi GuoState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Yi ZhengState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Yizhao ChangState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Fei GuState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Xuemei LuState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Qingsheng QiState Key Laboratory of Microbial Technology, Shandong university, Qingdao 266237, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Base editing (BE) is a promising genome engineering tool for modifying DNA or RNA and has been widely used in various microorganisms as well as eukaryotic cells. Despite the proximal protospacer adjacent motif (PAM) is critical to the targeting range and off-target effect of BE, there is still lack of a specific approach to analyze the PAM pattern in BE systems. Here, we developed a base editing-coupled survival screening method. Using dCas9 from

Indexed as

Molecular biologySystems biology

Identifiers

PMID34337358

DOI10.1016/j.isci.2021.102769

PMCPMC8324807

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.