Evidence map›Paper›PMID 34336708›Full record

ArticleFrontiers in cellular and infection microbiology2021

Rapid and Visual Detection of SARS-CoV-2 Using Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Linked With Gold Nanoparticle-Based Lateral Flow Biosensor.

Xu Chen, Qingxue Zhou, Shijun Li, Hao Yan, Bingcheng Chang, Yuexia Wang, Shilei Dong

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 43 citations in OpenAlex.

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  18. Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19.Frontiers in bioengineering and biotechnology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 6 institutions in 1 country.

Xu ChenThe Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Qingxue ZhouClinical Laboratory, Hangzhou Women's Hospital, Hangzhou, China.
Shijun LiLaboratory of Bacterial Infectious Disease of Experimental Centre, Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China.
Hao YanDepartment of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Bingcheng ChangThe Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Yuexia WangTCM Research Institute, Zhejiang Chinese Medical University, Hangzhou, China.
Shilei DongDepartment of Clinical Laboratory, Zhejiang Hospital, Hangzhou, China.
Guiyang College of Traditional Chinese Medicine · CNGuizhou Center for Disease Control and Prevention · CNHangzhou Women’s Hospital · CNZhejiang Center for Disease Control and Prevention · CNZhejiang Chinese Medical University · CNZhejiang Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has caused the outbreak of coronavirus disease 2019 (COVID-19) all over the world. In the absence of appropriate antiviral drugs or vaccines, developing a simple, rapid, and reliable assay for SARS-CoV-2 is necessary for the prevention and control of the COVID-19 transmission. Methods: A novel molecular diagnosis technique, named multiplex reverse transcription loop-mediated isothermal amplification, that has been linked to a nanoparticle-based lateral flow biosensor (mRT-LAMP-LFB) was applied to detect SARS-CoV-2 based on the SARS-CoV-2 Results: The SARS-CoV-2 specific primers based on the Conclusion: The mRT-LAMP-LFB technique developed in the current study is a simple, rapid, and reliable method with great specificity and sensitivity when it comes to identifying SARS-CoV-2 virus for prevention and control of the COVID-19 disease, especially in resource-constrained regions of the world.

Indexed as

Biosensing TechniquesCOVID-19Metal NanoparticlesGoldHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesReverse TranscriptionRNA, ViralSARS-CoV-2Sensitivity and SpecificityGoldRNA, ViralCOVID-19lateral flow biosensorlimit of detectionreverse transcription-loop-mediated isothermal amplificationSARS-CoV-2

Identifiers

PMID34336708
PMCPMC8316814
OpenAlexW3188840569

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.