Evidence map›Paper›PMID 34335782›Full record

ArticleInternational journal of microbiology2021

Isolation and Characterization of Urease-Producing Soil Bacteria.

Eshetu Mekonnen, Ameha Kebede, Asefa Nigussie, Gessese Kebede, Mesfin Tafesse

Open access · goldAbstract read
In one paragraph

Article in International journal of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 59 citations in OpenAlex.

  1. Isolation and characterization of ureolytic bacteria driving microbially induced calcite precipitation in cement contaminated soil.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
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  8. Genome sequences of aMicrobiology resource announcements · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Eshetu MekonnenDire Dawa University, College of Natural and Computational Sciences, Department of Biology, Dire Dawa, Ethiopia.ORCID https://orcid.org/0000-0001-5322-1294
Ameha KebedeHaramaya University, College of Natural and Computational Sciences, School of Biology and Biotechnology, Haramaya, Ethiopia.
Asefa NigussieAddis Ababa Science and Technology University, Department of Biotechnology, Addis Ababa, Ethiopia.
Gessese KebedeAddis Ababa Science and Technology University, Department of Biotechnology, Addis Ababa, Ethiopia.
Mesfin TafesseAddis Ababa Science and Technology University, Department of Biotechnology, Addis Ababa, Ethiopia.
Addis Ababa Science and Technology University · ETDire Dawa University · ETHaramaya University · ET

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urease is an enzyme produced by ureolytic microorganisms which hydrolyzes urea into ammonia and carbon dioxide. Microbial urease has wide applications in biotechnology, agriculture, medicine, construction, and geotechnical engineering. Urease-producing microbes can be isolated from different ecosystems such as soil, oceans, and various geological formations. The aim of this study was to isolate and characterize rapid urease-producing bacteria from Ethiopian soils. Using qualitative urease activity assay, twenty urease-producing bacterial isolates were screened and selected. Among these, three expressed urease at high rates as determined by a conductivity assay. The isolates were further characterized with respect to their biochemical, morphological, molecular, and exoenzyme profile characteristics. The active urease-producing bacterial isolates were found to be nonhalophilic to slightly halophilic neutrophiles and aerobic mesophiles with a range of tolerance towards pH (4.0-10.0), NaCl (0.25-5%), and temperature (20-40°C). According to the API ZYM assays, all three isolates were positive for alkaline phosphatase, leucine aryl amidase, acid phosphatase, and naphthol_AS_BI_phosphohydrolase. The closest described relatives of the selected three isolates (Isolate_3, Isolate_7, and Isolate_11) were

Identifiers

PMID34335782
PMCPMC8286177
OpenAlexW3182912477

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.