ArticleFrontiers in microbiology2021
Comparative Analysis of 16S rRNA Gene and Metagenome Sequencing in Pediatric Gut Microbiomes.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 2 of them syntheses that pooled it.
What it found
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Who cites it
101 citing papers in PubMed, 2 syntheses or guidelines pooled it, 169 citations in OpenAlex.
- Effect of Intensity and Duration of Exercise on Gut Microbiota in Humans: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Pooled it
- Effect of a complementary feeding intervention based on iron- and zinc-biofortified pearl millet on the gut microbiota in 12-18-month-old children: a randomized trial.Nature communications · 2026Trial
- Trial
- Small Intestinal Bacterial Overgrowth in the Pediatric Population: A Review of Pathophysiology, Diagnosis, and Management.Current gastroenterology reports · 2026Review
- Global analysis of infant gut microbiota revealed distinctive maturation dynamics across lifestyles.NPJ biofilms and microbiomes · 2026Article
- Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis.Microbiology spectrum · 2026Article
- Oral microbiota characteristics in children younger than 3 years with febrile seizures: a prospective observational study.BMC microbiology · 2026Observational
- Microbial Diversity of Spontaneously Fermented Camel Milk.Foods (Basel, Switzerland) · 2026Review
- Exploring the oral microbiome: from traditional techniques to advanced omics and databases.Antonie van Leeuwenhoek · 2026Review
- The microbial metabolome: remodeling the therapeutic landscape in hematologic malignancies.NPJ biofilms and microbiomes · 2026Review
- Interactions between host genetics and gut microbiome influence susceptibility to childhood asthma and lung function.The Journal of allergy and clinical immunology · 2026Article
- Urbanization shapes gut bacterial communities and metabolic functions in migratory wild birds across Bangladesh.BMC microbiology · 2026Article
- Applications and technical challenges of MALDI mass spectrometry imaging in microbial ecosystems and metabolic network research.Archives of microbiology · 2026Review
- Towards standardization in pig microbiome research based on a comprehensive twenty-year review.Animal microbiome · 2026Review
- Personalizing Nutritional Therapy in Pediatric Oncology: The Role of Gut Microbiome Profiling and Metabolomics in Mitigating Mucositis and Enhancing Immune Response to Chemotherapy.Children (Basel, Switzerland) · 2026Review
- Impact of maternal, infant, and household factors on early-life gut microbiome development in a rural setting.The ISME journal · 2026Article
- Phylogenetic Analysis and In Vitro Antimicrobial Potential of Some Herbal Extracts Against Multidrug-ResistantJournal of tropical medicine · 2026Article
- Extracellular vesicles ofGut microbes · 2025Article
- Dual-approach analysis of gut microbiome in patients with type 1 diabetes and diabetic kidney disease.Annals of medicine · 2025Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The colonization of the human gut microbiome begins at birth, and over time, these microbial communities become increasingly complex. Most of what we currently know about the human microbiome, especially in early stages of development, was described using culture-independent sequencing methods that allow us to identify the taxonomic composition of microbial communities using genomic techniques, such as amplicon or shotgun metagenomic sequencing. Each method has distinct tradeoffs, but there has not been a direct comparison of the utility of these methods in stool samples from very young children, which have different features than those of adults. We compared the effects of profiling the human infant gut microbiome with 16S rRNA amplicon vs. shotgun metagenomic sequencing techniques in 338 fecal samples; younger than 15, 15-30, and older than 30 months of age. We demonstrate that observed changes in alpha-diversity and beta-diversity with age occur to similar extents using both profiling methods. We also show that 16S rRNA profiling identified a larger number of genera and we find several genera that are missed or underrepresented by each profiling method. We present the link between alpha diversity and shotgun metagenomic sequencing depth for children of different ages. These findings provide a guide for selecting an appropriate method and sequencing depth for the three studied age groups.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.